Document Detail

B9-serine residue is crucial for insulin actions in glucose metabolism.
MedLine Citation:
PMID:  11749779     Owner:  NLM     Status:  MEDLINE    
AIM: To explore the importance of B9 and B10 amino acid residues in the insulin molecule. METHODS: The [B9Glu, B10Asp] insulin (E,D-insulin) receptor binding activity, glucose upta ke activity, and lipogenesis activity were measured in isolated adipocytes. The translocation of glucose transporter 4 (Glut4) and the phosphorylation of insulin receptor (IR) stimulated by E, D-insulin were determined by Western blotting. RESULTS: Compared with native insulin, the receptor binding activity of E,D-insulin was 31 %; the stimulating activity of E,D-insulin in glucose transport and lipogenesis were 45 % and 40 % respectively; the stimulations of Glut4 translocation and insulin receptor autophosphorylation of E,D-insulin were about 58 % and 46 % respectively. CONCLUSION: B9-serine residue is crucial for insulin actions in glucose metabolism.
Z Y Liao; Y H Tang; M H Xu; Y M Feng; S Q Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  22     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2003-10-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  939-43     Citation Subset:  IM    
Institute of Biochemistry, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
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MeSH Terms
Adipocytes / metabolism
Binding Sites
Cell Separation
Epididymis / metabolism
Glucose / metabolism*
Insulin / analogs & derivatives*,  chemistry,  metabolism,  pharmacology*
Monosaccharide Transport Proteins / metabolism
Rats, Sprague-Dawley
Receptor, Insulin / metabolism*
Serine / metabolism,  pharmacology*
Translocation, Genetic
Reg. No./Substance:
0/Monosaccharide Transport Proteins; 11061-68-0/Insulin; 110734-38-8/insulin, Asp(B9)-Glu(B27)-; 50-99-7/Glucose; 56-45-1/Serine; EC, Insulin

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