Document Detail


B56-associated protein phosphatase 2A is required for survival and protects from apoptosis in Drosophila melanogaster.
MedLine Citation:
PMID:  11997504     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein phosphorylation and specific protein kinases can initiate signal transduction pathways leading to programmed cell death. The specific protein phosphatases regulating apoptosis have been more elusive. Using double-stranded RNA-mediated interference (RNAi), the role of protein phosphatase 2A (PP2A) in cellular signaling was investigated. Knockdown of A or C subunits individually or of combined B subunits led to concurrent loss of nontargeted PP2A subunits, suggesting that PP2A is an obligate heterotrimer in vivo. Global knockdown of PP2A activity or specific loss of redundant B56 regulatory subunits caused cell death with the morphological and biochemical changes characteristic of apoptosis in cultured S2 cells. B56:PP2A-regulated apoptosis required caspases and the upstream regulators dark, reaper, head involution defective, and dp53. In Drosophila embryos, knockdown of B56-regulated PP2A activity resulted in apoptosis and failure of gastrulation, an effect that was blocked by concurrent RNAi of the caspase DRICE: B56-regulated PP2A activity appears to be required upstream of dp53 to maintain a critical proapoptotic substrate in a dephosphorylated, inactive state, thereby preventing apoptosis in Drosophila S2 cells.
Authors:
Xinghai Li; Anne Scuderi; Anthea Letsou; David M Virshup
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  22     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-08     Completed Date:  2002-06-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3674-84     Citation Subset:  IM    
Affiliation:
Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112-5550, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Base Sequence
Cell Line
Drosophila melanogaster / cytology*,  embryology,  enzymology*,  genetics
Genes, Insect
Models, Biological
Phosphoprotein Phosphatases / chemistry,  metabolism*
Protein Phosphatase 2
Protein Subunits
RNA, Double-Stranded / genetics,  metabolism
Signal Transduction
Grant Support
ID/Acronym/Agency:
P30 CA42014/CA/NCI NIH HHS; R01 CA80809/CA/NCI NIH HHS; R01GM61972/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Protein Subunits; 0/RNA, Double-Stranded; EC 3.1.3.16/Phosphoprotein Phosphatases; EC 3.1.3.16/Protein Phosphatase 2
Comments/Corrections

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