| B16-G4F mouse melanoma cells: an MSH receptor-deficient cell clone. | |
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MedLine Citation:
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PMID: 8482388 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The two mouse melanoma cell lines B16-F1 and B16-G4F retain their melanogenic capacity when cultured in vitro. Melanotropic peptides such as alpha-melanocyte-stimulating hormone (alpha-MSH) induce formation and release of melanin pigment in B16-F1 cells. In contrast, B16-G4F cells do not respond to alpha-MSH. Using receptor-binding analysis and photoaffinity crosslinking we demonstrate that the lack of response of B16-G4F cells to alpha-MSH is due to the absence of functional MSH receptors from the cell surface. Northern blot analysis of receptor mRNA revealed that MSH receptor mRNA is not expressed in B16-G4F cells. These cells represent a new tool for the study of signal pathways related to the control of melanogenesis in melanoma cells. |
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Authors:
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F F Solca; J Chluba-de Tapia; K Iwata; A N Eberle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 322 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1993 May |
Date Detail:
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Created Date: 1993-06-01 Completed Date: 1993-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 177-80 Citation Subset: IM |
Affiliation:
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Department of Research (ZLF), University Hospital, Basel, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Clone Cells Melanocyte-Stimulating Hormones / metabolism* Melanoma, Experimental / metabolism* Mice Molecular Sequence Data Receptors, Pituitary Hormone / metabolism* Tumor Cells, Cultured / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/MSH receptor; 0/Receptors, Pituitary Hormone; 9002-79-3/Melanocyte-Stimulating Hormones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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