Document Detail

B-myb alters the response of myeloid precursor cells to G-CSF.
MedLine Citation:
PMID:  10623475     Owner:  NLM     Status:  MEDLINE    
The human B-myb gene encodes a cell cycle-regulated DNA-binding phosphoprotein which functions as a transcription factor with an important role in cell cycle progression, survival, and differentiation. Recently, it has been demonstrated that ectopic murine B-myb expression blocked the ability of 32Dcl3 cells to proliferate in response to granulocyte colony-stimulating factor (G-CSF) and accelerated the induction of terminal differentiation. In contrast, we report that while 32Dcl3 cells overexpressing human B-myb do display some markers of myeloid differentiation earlier than parental cells, including the expression of myeloperoxidase mRNA and the appearance of band myelocytes in G-CSF-induced cultures, the induction of late markers of differentiation is inhibited. The expression of lactoferrin mRNA is absent and the appearance of terminally differentiated polymorphonuclear cells is severely impaired in B-myb-expressing 32Dcl3 cells. Furthermore, continuous exposure to G-CSF results in the outgrowth of a culture which expresses increased levels of B-myb RNA and is dependent on G-CSF for proliferation while retaining responsiveness to interleukin-3. These data suggest that the B-myb gene is involved in early transcriptional events during myeloid differentiation, but that its expression prevents terminal differentiation.
A Engelhard; K Campbell; B Calabretta
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  254     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-24     Completed Date:  2000-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  153-62     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Microbiology and Immunology, Kimmel Cancer Institute, Philadelphia, Pennsylvania, 19107, USA.
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MeSH Terms
CCAAT-Enhancer-Binding Proteins
Cell Cycle Proteins*
Cell Differentiation
Cell Division
Cell Line
Cell Survival
DNA-Binding Proteins / genetics,  metabolism*
Gene Expression
Granulocyte Colony-Stimulating Factor / metabolism*,  pharmacology
Hematopoietic Stem Cells / cytology*,  drug effects*,  metabolism
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Trans-Activators / biosynthesis,  genetics,  metabolism*
Transcription Factors / biosynthesis
Reg. No./Substance:
0/C-EPB transcription factor; 0/CCAAT-Enhancer-Binding Proteins; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/MYBL2 protein, human; 0/Mybl2 protein, mouse; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Trans-Activators; 0/Transcription Factors; 0/proto-oncogene protein Spi-1; 143011-72-7/Granulocyte Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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