| B lymphocytes in human subcutaneous adipose crown-like structures. | |
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MedLine Citation:
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PMID: 22395812 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Accumulation of macrophages and T cells within crown-like structures (CLS) in subcutaneous adipose tissue predicts disease severity in obesity-related insulin resistance (OIR). Although rodent data suggest the B cell is an important feature of these lesions, B cells have not been described within the human CLS. In order to identify B cells in the human subcutaneous CLS (sCLS) in obese subjects and determine whether the presence of B cells predict insulin resistance, we examined archived samples of subcutaneous and omental fat from 32 obese men and women and related findings to clinical parameters. Using immunohistochemistry, we identified B (CD19(+)) and T cells (CD3 (+)) within the sCLS and perivascular space. The presence and density of B cells (B cells per high-power field (pHPF), T cells pHPF, and B cell:T cell (B:T) ratio) were compared with measures of insulin resistance (homeostasis model assessment (HOMA)) and other variables. In 16 of 32 subjects (50%) CD19(+) B cells were localized within sCLS and were relatively more numerous than T cells. HOMA was not different between subjects with CD19(+) vs. CD19(-) sCLS (5.5 vs. 5.3, P = 0.88). After controlling for diabetes and glycemia (hemoglobin A(1c) (HbA(1c))), the B:T ratio correlated with current metformin treatment (r = 0.89, P = 0.001). These results indicate that in human OIR, B cells are an integral component of organized inflammation in subcutaneous fat, and defining their role will lead to a better understanding of OIR pathogenesis and potentially impact treatment. |
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Authors:
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Marie E McDonnell; Lisa M Ganley-Leal; Ankeeta Mehta; Sherman J Bigornia; Melanie Mott; Qasim Rehman; Melissa G Farb; Donald T Hess; Lija Joseph; Noyan Gokce; Caroline M Apovian |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-03-07 |
Journal Detail:
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Title: Obesity (Silver Spring, Md.) Volume: 20 ISSN: 1930-739X ISO Abbreviation: Obesity (Silver Spring) Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-26 Completed Date: 2012-10-17 Revised Date: 2013-05-03 |
Medline Journal Info:
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Nlm Unique ID: 101264860 Medline TA: Obesity (Silver Spring) Country: United States |
Other Details:
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Languages: eng Pagination: 1372-8 Citation Subset: IM |
Affiliation:
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Department of Medicine and Section of Endocrinology, Diabetes, Nutrition and Weight Management, Boston University School of Medicine, Boston, MA, USA. marie.mcdonnell@bmc.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD19 / metabolism B-Lymphocytes / immunology, pathology* Blood Glucose / metabolism* Body Mass Index Female Hemoglobin A, Glycosylated / metabolism* Humans Immunohistochemistry Insulin Resistance Male Obesity / immunology, pathology* Omentum / immunology, pathology* Predictive Value of Tests Subcutaneous Fat / pathology* |
| Grant Support | |
ID/Acronym/Agency:
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DK046200/DK/NIDDK NIH HHS; P01 HL081587-02/HL/NHLBI NIH HHS; P30 DK046200-08/DK/NIDDK NIH HHS; P30 DK046200-10/DK/NIDDK NIH HHS; R01 HL084213-01A1/HL/NHLBI NIH HHS; R01 HL114675/HL/NHLBI NIH HHS; UL1RR025771/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD19; 0/Blood Glucose; 0/Hemoglobin A, Glycosylated; 0/hemoglobin A1c protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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