Document Detail


B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40.
MedLine Citation:
PMID:  7512028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of CD40 ligand (CD40L) on activated T cells (CD4+ T cell clone MT9) is diminished when the T cells are cultured in the presence of B cells. This effect, observed both with normal tonsil B cells and with the B cell line JY, was detected after 6 h and sustained at least until 18 h of co-culture. Analysis of mRNA showed that CD40L mRNA levels were not modified after 6 h, but were significantly down-regulated after 18 h of co-culture with B cells. Although CD40L expression could not be detected by a CD40-Fc chimera, the molecule was still expressed at the membrane as shown with a polyclonal antiserum against CD40L (anti-TRAP). In addition, T cells activated in the presence of B cells were stained by a polyclonal antiserum against CD40, without the appearance of CD40 mRNA. These results indicated that a soluble form of CD40 (sCD40) bound to the expressed CD40L on T cells. The existence of sCD40 was confirmed by detection of sCD40 in B cell supernatants using a specific enzyme-linked immunosorbent assay. Collectively, these data show that B cells can regulate the expression of CD40L on activated T cells at least by two different mechanisms.
Authors:
C van Kooten; C Gaillard; J P Galizzi; P Hermann; F Fossiez; J Banchereau; D Blanchard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  24     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-05-09     Completed Date:  1994-05-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  787-92     Citation Subset:  IM    
Affiliation:
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / metabolism*
Antigens, CD40
Antigens, Differentiation, B-Lymphocyte / metabolism*
B-Lymphocytes / physiology*
CD40 Ligand
Cells, Cultured
Down-Regulation
Humans
Lymphocyte Activation
Membrane Glycoproteins / analysis*
RNA, Messenger / analysis*
T-Lymphocytes / metabolism*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD40; 0/Antigens, Differentiation, B-Lymphocyte; 0/Membrane Glycoproteins; 0/RNA, Messenger; 147205-72-9/CD40 Ligand

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