Document Detail

B-cells purified using azide give different responses in vitro to B-cells purified without azide.
MedLine Citation:
PMID:  2438214     Owner:  NLM     Status:  MEDLINE    
Some established techniques for B-cell enrichment include azide in the media. Here, B-cells were enriched using a technique which transiently exposed the cells of 15 mM sodium azide. After 3-4 days in culture, the precursor frequency of autoantibody-secreting cells in this population was 0.113%. In contrast, when B-cells were enriched using a technique which eliminated their exposure to sodium azide, the number of autoantibody-secreting precursor cells detected in cultures was increased up to 20-fold. Therefore, it is concluded that transient exposure to azide affects functional activities of B-cells in vitro.
S Daenke; K O Cox
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology letters     Volume:  14     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-06-26     Completed Date:  1987-06-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  331-3     Citation Subset:  IM    
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MeSH Terms
Autoantibodies / secretion*
Azides / pharmacology*
B-Lymphocytes / drug effects,  immunology*
Cell Separation / methods*
Dextran Sulfate
Dextrans / pharmacology
Lipopolysaccharides / pharmacology
Sodium Azide
Reg. No./Substance:
0/Autoantibodies; 0/Azides; 0/Lipopolysaccharides; 26628-22-8/Sodium Azide; 9004-54-0/Dextrans; 9042-14-2/Dextran Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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