Document Detail


B-cell leukemia protein-2 and peptide YY chemotherapy resistance in colon cancer.
MedLine Citation:
PMID:  10612539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Inhibition of apoptosis may allow cells with drug-induced damage to escape programmed cell death. The bcl-2 protein inhibits apoptosis and bcl-2 overexpression has been associated with drug resistance. It is our hypothesis that higher levels of bcl-2 expression will be seen in colon cancer cells resistant to PYY treatment. METHODS: Caco2 and HCT116 colon cancer cells were treated with 2 microM PYY for 24 hours. Protein was extracted from cells surviving PYY treatment; bcl-2 expression was measured by enzyme-linked immunosorbent assay (ELISA) and confirmed by Western blotting. RESULTS: Caco2 and HCT116 cells surviving PYY treatment demonstrated increased bcl-2 from 20.54+/-2.7 to 28.63+/-2.20 units/mL (P <0.05) and 21.98+/-1.28 to 29.32*+/-2.26 units/mL, respectively. CONCLUSIONS: Increased expression of bcl-2 is seen in a population of colon cancer cells resistant to PYY. Hence, bcl-2 may protect neoplastic cells from apoptosis; its levels may be useful in predicting chemotherapy response and in selecting appropriate drug regimens.
Authors:
K Kling; F Kim; M Cole; D McFadden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of surgery     Volume:  178     ISSN:  0002-9610     ISO Abbreviation:  Am. J. Surg.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-01-05     Completed Date:  2000-01-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370473     Medline TA:  Am J Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  411-4     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, University of California, Los Angeles, USA.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Blotting, Western
Cell Survival
Colonic Neoplasms / drug therapy,  genetics*
Enzyme-Linked Immunosorbent Assay
Genes, bcl-2 / genetics*
Humans
Peptide YY / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / biosynthesis*,  genetics
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 106388-42-5/Peptide YY

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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