| B-cell leukemia protein-2 and peptide YY chemotherapy resistance in colon cancer. | |
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MedLine Citation:
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PMID: 10612539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Inhibition of apoptosis may allow cells with drug-induced damage to escape programmed cell death. The bcl-2 protein inhibits apoptosis and bcl-2 overexpression has been associated with drug resistance. It is our hypothesis that higher levels of bcl-2 expression will be seen in colon cancer cells resistant to PYY treatment. METHODS: Caco2 and HCT116 colon cancer cells were treated with 2 microM PYY for 24 hours. Protein was extracted from cells surviving PYY treatment; bcl-2 expression was measured by enzyme-linked immunosorbent assay (ELISA) and confirmed by Western blotting. RESULTS: Caco2 and HCT116 cells surviving PYY treatment demonstrated increased bcl-2 from 20.54+/-2.7 to 28.63+/-2.20 units/mL (P <0.05) and 21.98+/-1.28 to 29.32*+/-2.26 units/mL, respectively. CONCLUSIONS: Increased expression of bcl-2 is seen in a population of colon cancer cells resistant to PYY. Hence, bcl-2 may protect neoplastic cells from apoptosis; its levels may be useful in predicting chemotherapy response and in selecting appropriate drug regimens. |
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Authors:
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K Kling; F Kim; M Cole; D McFadden |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: American journal of surgery Volume: 178 ISSN: 0002-9610 ISO Abbreviation: Am. J. Surg. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-01-05 Completed Date: 2000-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370473 Medline TA: Am J Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 411-4 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, University of California, Los Angeles, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Blotting, Western Cell Survival Colonic Neoplasms / drug therapy, genetics* Enzyme-Linked Immunosorbent Assay Genes, bcl-2 / genetics* Humans Peptide YY / pharmacology* Proto-Oncogene Proteins c-bcl-2 / biosynthesis*, genetics Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 106388-42-5/Peptide YY |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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