Document Detail


B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells.
MedLine Citation:
PMID:  19713459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Afferent lymph is transported throughout lymph nodes (LNs) by the conduit system. Whereas this conduit network is dense in the T-cell zone, it is sparse in B-cell follicles. In this study, we show that this differential organization emerges during lymph node development. Neonatal LNs lack B follicles, but have a developed T-cell zone and a dense conduit network. As new T and B cells enter the developing LN, the conduit network density is maintained in the T, but not the B zone, leading to a profound remodeling of the follicular network that nevertheless maintains its connectivity. In adults, the residual follicular conduits transport soluble antigen to deep regions, where follicular dendritic cells are abundant and appear to replace the fibroblastic reticular cells that enwrap conduits in the T zone. This strategic location correlates with the capacity of the follicular dendritic cells to capture antigen even in the absence of antigen-specific antibodies. Together, these results describe how the stromal organization of the T and B regions of LNs diverges during development, giving rise to distinct antigen transport and delivery modes in the 2 compartments.
Authors:
Marc Bajénoff; Ronald N Germain
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2009-08-27
Journal Detail:
Title:  Blood     Volume:  114     ISSN:  1528-0020     ISO Abbreviation:  Blood     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-07     Completed Date:  2010-01-05     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4989-97     Citation Subset:  AIM; IM    
Affiliation:
Centre National de la Recherche Scientifique, Inserm, Unité 924, Université de Nice-Sophia Antipolis, Valbonne, France. bajenoff@ciml.univ-mrs.fr
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MeSH Terms
Descriptor/Qualifier:
Adoptive Transfer
Animals
Antigen Presentation / immunology
B-Lymphocytes / cytology,  immunology*
Dendritic Cells, Follicular / cytology,  immunology*
Fluorescent Antibody Technique
Lymph Nodes / anatomy & histology*,  immunology*
Mice
Mice, Inbred C57BL
Mice, Transgenic
T-Lymphocytes / cytology,  immunology
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