| B-Z transition of poly(dG-m5dC).poly(dG-m5dC) in the presence of basic oligopeptides. | |
| | |
MedLine Citation:
|
PMID: 8329326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of basic oligopeptides (Lys-Ala-Ala)n and (Lys-Leu-Ala)n (n = 1-4) on the B-Z transition of poly(dG-m5dC).poly(dG-m5dC) in aqueous solution and in methanol-water mixtures was investigated by c.d. spectroscopy. In aqueous solution dimers and higher oligomers induce a transition to the Z conformation. These temperature dependent transitions are consistent with positive delta HB-Z values which depend on peptide composition. In the absence of peptides no transition can be observed. In the presence of peptides B-Z transition can be induced by small amount of methanol. The temperature dependence of this transition is consistent with a small, but definitely negative delta HB-Z. At high methanol concentration a transition to Z' type conformation was observed. In subcritical methanol concentrations B-Z transition can be induced by the addition of peptides. In this case the delta HB-Z values are again very small, but definitely positive. The effects of bulky hydrophobic peptide side chains, of the presence of methanol and the temperature dependences are consistent with an important contribution of hydrophobic interactions in maintaining the stability of the Z DNA-peptide complex. |
| | |
Authors:
|
H Votavová; J Sponar |
Related Documents
:
|
2101836 - Hb davenport or alpha 2(78)(ef7)asn----his beta 2. 23510146 - Peptides:β-cyclodextrin inclusion compound as higher effective antimicrobial and anti-... 3838976 - A new unstable, high oxygen affinity hemoglobin: hb nagoya or beta 97 (fg4) his----pro. 23469146 - The c-terminal region mesd peptide mimics full-length mesd and acts as an inhibitor of ... 6134616 - Interaction between the different domains of aminoacyl-trna and the elongation-factor-t... 18556796 - Expression, purification, and structural characterization of cfra, a putative iron tran... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: International journal of biological macromolecules Volume: 15 ISSN: 0141-8130 ISO Abbreviation: Int. J. Biol. Macromol. Publication Date: 1993 Jun |
Date Detail:
|
Created Date: 1993-08-19 Completed Date: 1993-08-19 Revised Date: 2000-12-18 |
Medline Journal Info:
|
Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 139-44 Citation Subset: IM |
Affiliation:
|
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Circular Dichroism Molecular Sequence Data Nucleic Acid Conformation* Oligopeptides / chemistry* Polydeoxyribonucleotides / chemistry* Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Oligopeptides; 0/Polydeoxyribonucleotides; 29855-95-6/poly(dC-dG) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Exposure dependent increase in DNA single strand breaks in leucocytes from workers exposed to low co...
Next Document: Solution and solid-state circular dichroism analyses of a human salivary proline-rich glycoprotein r...