Document Detail


B-Myc, a proximal caput epididymal protein, is dependent on androgens and testicular factors for expression.
MedLine Citation:
PMID:  11369584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myc family of transcriptional regulators carries out critical roles in the control of cellular proliferation, differentiation, apoptosis, and tumorigenesis. The B-myc gene is a recently identified myc family member that has not been well characterized. Previously, we have shown that B-Myc inhibits the ability of c-Myc to transform cells and can inhibit cellular proliferation. Because B-myc is primarily expressed in hormonally regulated tissues with predominant expression in the epididymis, we examined in greater detail B-myc expression in the epididymis to ultimately understand potential roles B-myc may play in this and other hormonally regulated tissues. Herein we demonstrate that, in contrast to c-myc, B-myc mRNA and protein expression are highly regionalized with expression predominantly in the proximal caput epididymal region. Furthermore, in situ and immunohistochemical analyses show that within the epididymis B-myc mRNA and protein are specifically expressed by the epithelial cells and that B-Myc protein is localized to both the nuclear and cytosolic compartments. Castration and hormone replacement studies further show that expression of the B-myc mRNA is highly dependent on the presence of androgens and testicular factors. Finally, mRNA turnover studies demonstrate that the B-myc mRNA is relatively unstable with a half-life of 3.5 h. Taken together, the highly restricted and regulated expression of the B-myc gene suggests it may play important regulatory roles in the epididymis and perhaps other hormonally regulated tissues.
Authors:
G A Cornwall; R Collis; Q Xiao; N Hsia; S R Hann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  64     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-08-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1600-7     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA. gail.cornwall@ttmc.ttuhsc.edu
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MeSH Terms
Descriptor/Qualifier:
Androgens / pharmacology*
Animals
Blotting, Northern
Cell Line
Dactinomycin / pharmacology
Drug Stability
Epididymis / chemistry,  metabolism*
Female
Gene Expression / drug effects*
Immunohistochemistry
In Situ Hybridization
Male
Mice
Mice, Inbred ICR
Nucleic Acid Synthesis Inhibitors / pharmacology
Proto-Oncogene Proteins c-myc / analysis,  genetics*
RNA, Messenger / analysis
Testis / metabolism*
Grant Support
ID/Acronym/Agency:
CA47399/CA/NCI NIH HHS; CA78888/CA/NCI NIH HHS; HD33903/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Androgens; 0/Bmyc protein, mouse; 0/Nucleic Acid Synthesis Inhibitors; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 50-76-0/Dactinomycin

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