Document Detail


B-Myb, a repressed trans-activating protein.
MedLine Citation:
PMID:  9428611     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
B-Myb belongs to a family of related transcription factors which share a unique DNA binding domain. B-Myb plays an important role in regulation of the cell cycle. Its expression is upregulated by the human papilloma virus HPV16 E7 oncoprotein. Overexpression of B-Myb can bypass p53-mediated cell cycle arrest. The founding member of the myb gene family, c-Myb, and A-Myb are involved in hematopoiesis and neurogenesis, respectively, and are both activators of gene transcription. Whether B-Myb is a transactivator or a repressor, however, has remained a matter of discussion. We reviewed the transactivation potential of B-Myb in yeast, taking advantage of the fact that inducible gene activation is an evolutionarily conserved process. By mutational analysis we localized a conserved activation domain in B-Myb. In vertebrate cells the transactivation potential of B-Myb is concealed by the C-terminal part of the protein. We show that the cell cycle regulators cyclin A and cyclin E activate B-Myb by eradicating the inhibition mediated by its carboxy-terminus. Our data suggest that in vertebrates the trans-activating function of B-Myb is regulated during the cell cycle and link Myb functions to cell cycle progression.
Authors:
S Ansieau; E Kowenz-Leutz; R Dechend; A Leutz
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of molecular medicine (Berlin, Germany)     Volume:  75     ISSN:  0946-2716     ISO Abbreviation:  J. Mol. Med.     Publication Date:    1997 Nov-Dec
Date Detail:
Created Date:  1998-02-09     Completed Date:  1998-02-09     Revised Date:  2011-07-08    
Medline Journal Info:
Nlm Unique ID:  9504370     Medline TA:  J Mol Med (Berl)     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  815-9     Citation Subset:  IM    
Affiliation:
Max Delbrück Center for Molecular Medicine, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Cycle / genetics
Cell Cycle Proteins*
Cell Division / genetics
Chickens
DNA-Binding Proteins / genetics,  physiology*
Gene Expression Regulation / drug effects
Humans
Mice
Molecular Sequence Data
Sequence Homology, Amino Acid
Trans-Activators / genetics,  physiology*
Transcription Factors / genetics,  physiology*
Transcriptional Activation
Xenopus
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/MYBL2 protein, human; 0/Mybl2 protein, mouse; 0/Trans-Activators; 0/Transcription Factors

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