Document Detail


B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1).
MedLine Citation:
PMID:  23032261     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
B-Myb is a highly conserved member of the Myb transcription factor family, which plays an essential role in cell cycle progression by regulating the transcription of genes at the G 2/M-phase boundary. The role of B-Myb in other parts of the cell cycle is less well-understood. By employing siRNA-mediated silencing of B-Myb expression, we found that B-Myb is required for efficient entry into S-phase. Surprisingly, a B-Myb mutant that lacks sequence-specific DNA-binding activity and is unable to activate transcription of B-Myb target genes is able to rescue the S-phase defect observed after B-Myb knockdown. Moreover, we have identified polymerase delta-interacting protein 1 (Pdip1), a BTB domain protein known to bind to the DNA replication and repair factor PCNA as a novel B-Myb interaction partner. We have shown that Pdip1 is able to interact with B-Myb and PCNA simultaneously. In addition, we found that a fraction of endogenous B-Myb can be co-precipitated via PCNA, suggesting that B-Myb might be involved in processes related to DNA replication or repair. Taken together, our work suggests a novel role for B-Myb in S-phase that appears to be independent of its sequence-specific DNA-binding activity and its ability to stimulate the expression of bona fide B-Myb target genes.
Authors:
Eugen Werwein; Thore Schmedt; Heiko Hoffmann; Clemens Usadel; Nora Obermann; Jeffrey D Singer; Karl-Heinz Klempnauer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-03
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  11     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-04-23     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4047-58     Citation Subset:  IM    
Affiliation:
Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle Proteins / antagonists & inhibitors,  genetics,  metabolism*
Cell Line
Chickens
DNA / chemistry,  metabolism*
DNA Replication
HEK293 Cells
Hep G2 Cells
Humans
Mutation
Nuclear Proteins / metabolism*
Proliferating Cell Nuclear Antigen / metabolism
Protein Binding
RNA Interference
RNA, Small Interfering / metabolism
S Phase Cell Cycle Checkpoints
Trans-Activators / antagonists & inhibitors,  genetics,  metabolism*
Transfection
Grant Support
ID/Acronym/Agency:
5R01GM082940/GM/NIGMS NIH HHS; R01 GM082940/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/KCTD13 protein, human; 0/MYBL2 protein, human; 0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen; 0/RNA, Small Interfering; 0/Trans-Activators; 9007-49-2/DNA
Comments/Corrections

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