Document Detail


B-Myb expression in vascular smooth muscle cells occurs in a cell cycle-dependent fashion and down-regulates promoter activity of type I collagen genes.
MedLine Citation:
PMID:  8631934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The members of the Myb family of transcription factors are defined by homology in the DNA-binding domain; all bind the Myb-binding site (MBS) sequence (YG(A/G)C(A/C/G)GTT(G/A)). Here we report that cultured bovine vascular smooth muscle cells (SMCs) express B-myb. Levels of B-myb RNA found in exponential growth were reduced dramatically in serum-deprived quiescent SMCs; B-myb mRNA levels increased in the cell cycle during the late G1 to S phase transition following restimulation with serum, epidermal growth factor, or phorbol ester plus insulin-like growth factor-1. Changes in the rate of B-myb gene transcription could account for part of the observed increase following serum addition. Treatment of SMC cultures with actinomycin D indicated a >4-h half-life for B-myb mRNA during the S phase of the cell cycle. Cotransfection of either a bovine or human B-myb expression vector down-regulated the activity of a multimerized MBS element-driven reporter construct in SMCs. Putative MBS elements were detected upstream of the promoters of the two chains of type I collagen, which we have found to be expressed inversely with growth state of the SMC (Kindy, M. S., Chang, C.-J., and Sonenshein, G. E. (1988) J. Biol. Chem. 263, 11426-11430). In cotransfection experiments, B-myb expression down-regulated the promoter activity of alpha1(I) and alpha2(I) collagen constructs an average of 92 and 82%, respectively. Thus, B-myb represents a potential link in the observed inverse relationship between collagen gene expression and growth of vascular SMCs.
Authors:
D J Marhamati; G E Sonenshein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-07-02     Completed Date:  1996-07-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3359-65     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta
Base Sequence
Binding Sites
Cattle
Cell Cycle*
Cell Cycle Proteins*
Cells, Cultured
Collagen / biosynthesis*,  genetics*
Culture Media, Serum-Free
DNA-Binding Proteins / biosynthesis*,  metabolism
Dactinomycin / pharmacology
Epidermal Growth Factor / pharmacology
Female
G1 Phase
Gene Expression Regulation* / drug effects
Humans
Insulin-Like Growth Factor I / pharmacology
Kinetics
Molecular Sequence Data
Muscle, Smooth, Vascular / cytology,  metabolism*
Oligodeoxyribonucleotides
Promoter Regions, Genetic*
RNA, Messenger / metabolism
Recombinant Proteins / biosynthesis
S Phase
Tetradecanoylphorbol Acetate / pharmacology
Trans-Activators*
Transcription Factors / biosynthesis*,  metabolism
Transcription, Genetic / drug effects
Transfection
Grant Support
ID/Acronym/Agency:
HL07429/HL/NHLBI NIH HHS; HL13262/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Culture Media, Serum-Free; 0/DNA-Binding Proteins; 0/MYBL2 protein, human; 0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Trans-Activators; 0/Transcription Factors; 16561-29-8/Tetradecanoylphorbol Acetate; 50-76-0/Dactinomycin; 62229-50-9/Epidermal Growth Factor; 67763-96-6/Insulin-Like Growth Factor I; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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