| B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus. | |
| | |
MedLine Citation:
|
PMID: 21441451 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Follicular Th (T(FH)) cells are specialized in provision of help to B cells that is essential for promoting protective Ab responses. CD28/B7 (B7-1 and B7-2) interactions are required for germinal center (GC) formation, but it is not clear if they simply support activation of naive CD4 T cells during initiation of responses by dendritic cells or if they directly control T(FH) cells and/or directly influence follicular B cell differentiation. Using a model of vaccinia virus infection, we show that B7-2 but not B7-1 deficiency profoundly impaired T(FH) cell development but did not affect CD4 T cell priming and Th1 differentiation. Consistent with this, B7-2 but not B7-1 was required for acquisition of GC B cell phenotype, plasma cell generation, and virus-specific neutralizing Ab responses. Mixed adoptive transfer experiments indicated that bidirectional interactions between CD28 expressed on activated T cells and B7-2 expressed on follicular B cells were essential for maintenance of the T(FH) phenotype and GC B cell development. Our data provide new insight into the source and nature of molecules required for T(FH) cells to direct GC B cell responses. |
| | |
Authors:
|
Samira Salek-Ardakani; Youn Soo Choi; Mohammed Rafii-El-Idrissi Benhnia; Rachel Flynn; Ramon Arens; Stephen Shoenberger; Shane Crotty; Michael Croft; Shahram Salek-Ardakani |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-03-25 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 186 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-04-20 Completed Date: 2011-07-05 Revised Date: 2012-05-02 |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 5294-303 Citation Subset: AIM; IM |
Affiliation:
|
Division of Immune Regulation, La Jolla Institute for Allergy and Immunology, San Diego, CA 92037, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adoptive Transfer Animals Antigens, CD28 / biosynthesis, immunology Antigens, CD86 / biosynthesis, immunology* B-Lymphocytes / cytology, immunology*, metabolism Cell Differentiation / immunology Cell Separation Female Flow Cytometry Germinal Center / cytology, immunology Lymphocyte Activation / immunology* Mice Mice, Inbred C57BL Mice, Knockout Plasma Cells / cytology, immunology Spleen / cytology, immunology T-Lymphocytes, Helper-Inducer / immunology* Vaccinia / immunology* Vaccinia virus / immunology |
| Grant Support | |
ID/Acronym/Agency:
|
AI63107/AI/NIAID NIH HHS; AI67341/AI/NIAID NIH HHS; AI72543/AI/NIAID NIH HHS; AI77079/AI/NIAID NIH HHS; CA91837/CA/NCI NIH HHS; R01 AI063107-07/AI/NIAID NIH HHS; R01 AI067341-05/AI/NIAID NIH HHS; R01 AI072543-05/AI/NIAID NIH HHS; R01 CA091837-09/CA/NCI NIH HHS; R21 AI077079-02/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD28; 0/Antigens, CD86 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Host Defense Peptide LL-37 Selectively Reduces Proinflammatory Macrophage Responses.
Next Document: A multifunctional element in the mouse Ig? locus that specifies repertoire and Ig loci subnuclear lo...