Document Detail


B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family.
MedLine Citation:
PMID:  8570175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new member of the ATF/CREB family of transcription factors, called B-ATF, has been isolated from a cDNA library prepared from Epstein-Barr virus stimulated human B cells. B-ATF is a 125 amino acid nuclear protein possessing a basic leucine zipper domain that is most similar to the basic leucine zipper of ATF-3. Northern blot analysis of polyadenylated mRNA isolated from a variety of human tissues and established cell lines indicates that the 1.0 kilobase B-ATF mRNA is expressed differentially, with the strongest hybridization detected in lung and in Raji Burkitt's lymphoma. Efficient homodimerization of the B-ATF protein cannot be detected using the yeast two hybrid system or using in vitro binding assays with glutathione-s-transferase-B-ATF and maltose binding protein-B-ATF fusion proteins produced in E. coli. However, a yeast two hybrid library screen has identified the human oncoprotein JunB as a specific binding partner for B-ATF. Glutathione-s-transferase-B-ATF heterodimerizes efficiently with in vitro translated JunB, c-Jun, and JunD, but only weakly associates with c-Fos. In addition, electrophoretic mobility shift assays demonstrate that a B-ATF/c-Jun protein complex can interact with DNA containing a consensus binding site for AP-1, suggesting that B-ATF functions as a tissue-specific modulator of the AP-1 transcription complex in human cells.
Authors:
M J Dorsey; H J Tae; K G Sollenberger; N T Mascarenhas; L M Johansen; E J Taparowsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  11     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-03-01     Completed Date:  1996-03-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2255-65     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U15460
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Basic-Leucine Zipper Transcription Factors
Binding Sites
Biopolymers
DNA, Complementary
DNA-Binding Proteins*
Humans
Molecular Sequence Data
Nuclear Proteins / genetics,  metabolism*
Saccharomyces cerevisiae / genetics
Sequence Homology, Amino Acid
Transcription Factor AP-1 / metabolism*
Transcription Factors / genetics,  metabolism*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
F32-CA61635/CA/NCI NIH HHS; R01-CA42835/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/BATF protein, human; 0/Basic-Leucine Zipper Transcription Factors; 0/Biopolymers; 0/DNA, Complementary; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Transcription Factor AP-1; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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