Document Detail


B-1 cells express transgelin 2: unexpected lymphocyte expression of a smooth muscle protein identified by proteomic analysis of peritoneal B-1 cells.
MedLine Citation:
PMID:  16487589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
B-1 cells constitute a unique B cell subset that differs phenotypically, biochemically, and functionally from the predominant population of conventional B-2 cells. Functional differences include constitutive secretion of natural immunoglobulin and failure of BCR signaling to initiate proliferation. The origin of these differences remains uncertain. We hypothesized that unbiased analysis of differences in protein expression between highly pure populations of B-1 and B-2 cells might provide information not readily available through other means. To pursue this, we undertook 2D gel analysis of B-1 and B-2 cells combined with mass spectrometry. We identified the smooth muscle protein, transgelin 2, in peritoneal (but not splenic) B-1 cells and did not find it in splenic B-2 cells; these results were confirmed by Western blot analysis, which showed a more than 60-fold difference in transgelin 2 expression between peritoneal B-1 and splenic B-2 cells. In contrast, levels of transgelin 2 RNA differed to a much lesser extent (3-fold) in the two B cell populations, so transgelin 2 is an example of a molecule whose subset-specific expression is more readily detected by proteomic than transcriptomic analyses. Finally, transgelin 2 protein expression was induced in splenic B-2 cells; thus, transgelin 2 joins a number of other inducible molecules that are constitutively expressed by peritoneal B-1 but not splenic B-2 cells. Although the role of transgelin 2 in B-1 cell function remains uncertain, identification of this molecule demonstrates the value of examining protein expression in this B cell subset.
Authors:
Rubén Francés; Joseph R Tumang; Hiroaki Kaku; Sean M Gurdak; Thomas L Rothstein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-02-17
Journal Detail:
Title:  Molecular immunology     Volume:  43     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-03     Completed Date:  2006-08-25     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2124-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocyte Subsets / immunology,  metabolism*
Blotting, Western
Gene Expression Regulation* / immunology
Male
Mice
Mice, Inbred BALB C
Microfilament Proteins / biosynthesis*,  immunology
Muscle Proteins / biosynthesis*,  immunology
Muscle, Smooth / immunology,  metabolism*
Organ Specificity / immunology
Peritoneum / immunology,  metabolism*
Proteomics
Spleen / immunology,  metabolism
Grant Support
ID/Acronym/Agency:
AI29690/AI/NIAID NIH HHS; AI60896/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Microfilament Proteins; 0/Muscle Proteins; 0/transgelin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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