Document Detail


Azidothymidine induces dose dependent increase in micronuclei formation in cultured HeLa cells.
MedLine Citation:
PMID:  11446172     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exposure of HeLa cells to azidothymidine (AZT) resulted in a concentration dependent decline in growth kinetics. 100 microM of AZT completely inhibited the cell growth. The frequency of binucleate and multinucleate cells declined with increasing concentration of AZT and the formation of multinucleate cells was completely inhibited at 20 and 30 h at higher concentrations indicating inhibition of cell division. Similarly, the clonogenicity of cells declined in a concentration dependent manner and 10 microM AZT killed 50% of the cells. Conversely, the frequency of MNBNC (micronucleated binucleate cell) increased in a concentration dependent manner and was significantly higher in the AZT treated group than the non-drug treated control group. The relationship between concentrations of AZT and micronuclei-induction was linear for all the post-treatment time periods studied. The biological response was also determined by plotting the surviving fraction of cells on the X-axis and the number of micronuclei on the Y-axis. A close and inverse correlation between the surviving fraction and micronuclei formation was observed and the data could be fitted on to a linear quadratic model.
Authors:
R Aruna; G C Jagetia
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Die Pharmazie     Volume:  56     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-11     Completed Date:  2001-08-02     Revised Date:  2007-01-29    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  492-500     Citation Subset:  IM    
Affiliation:
Department of Radiobiology, Kasturba Medical College, Manipal, India.
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MeSH Terms
Descriptor/Qualifier:
Antimetabolites, Antineoplastic / pharmacology*
Cell Division / drug effects
Cell Survival / drug effects
Hela Cells
Humans
Kinetics
Micronucleus Tests*
Tumor Stem Cell Assay
Zidovudine / pharmacology*
Chemical
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 30516-87-1/Zidovudine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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