Document Detail

Azidothymidine (AZT) as a potential modifier of radiation response in vitro.
MedLine Citation:
PMID:  7718259     Owner:  NLM     Status:  MEDLINE    
The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 microM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned. For HeLa the effect was significant at 24 h. Cell-cycle analysis showed a significant accumulation in S-phase at 72 h for WiDr. For HeLa there was a significant accumulation in S-phase at 48 h. We conclude that under the reported experimental conditions, AZT as a thymidine analogue seems to reduce the cytotoxic effect of irradiation.
M L Copaceanu; P A Coucke; E Cottin; N Paschoud; R O Mirimanoff
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta oncologica (Stockholm, Sweden)     Volume:  34     ISSN:  0284-186X     ISO Abbreviation:  Acta Oncol     Publication Date:  1995  
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2009-05-12    
Medline Journal Info:
Nlm Unique ID:  8709065     Medline TA:  Acta Oncol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  213-8     Citation Subset:  IM    
Department of Radiation Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland.
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MeSH Terms
Cell Cycle / drug effects,  radiation effects
Dose-Response Relationship, Radiation
Hela Cells / drug effects,  metabolism,  radiation effects
Immunologic Factors
Radiation-Protective Agents / pharmacology*
Tumor Cells, Cultured / drug effects*,  metabolism,  radiation effects*
Zidovudine / pharmacology*
Reg. No./Substance:
0/Immunologic Factors; 0/Radiation-Protective Agents; 30516-87-1/Zidovudine

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