Document Detail

Azidothymidine (AZT) as a potential modifier of radiation response in vitro.
MedLine Citation:
PMID:  7718259     Owner:  NLM     Status:  MEDLINE    
The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 microM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned. For HeLa the effect was significant at 24 h. Cell-cycle analysis showed a significant accumulation in S-phase at 72 h for WiDr. For HeLa there was a significant accumulation in S-phase at 48 h. We conclude that under the reported experimental conditions, AZT as a thymidine analogue seems to reduce the cytotoxic effect of irradiation.
M L Copaceanu; P A Coucke; E Cottin; N Paschoud; R O Mirimanoff
Related Documents :
19156599 - Cytotoxic terpenoids from the fruits of vitex trifolia l.
10335869 - L20b cells simplify culture of polioviruses from clinical samples.
20352869 - Mechanism for alpha-mno2 nanowire-induced cytotoxicity in hela cells.
22441439 - Radiosensitizing effect of the novel hsp90 inhibitor nvp-auy922 in human tumour cell li...
22552949 - N-glycan biosynthesis inhibitors induce in vitro anticancer activity in colorectal canc...
16735119 - Preparation of chitosan-copper complexes and their antitumor activity.
21640429 - How do i begin? sensing extracellular stress to maintain yeast cell wall integrity.
23926339 - 6-o- and n-sulfated syndecan-1 promotes baculovirus binding and entry into mammalian ce...
18759049 - Cloned goats (capra hircus) from adult ear cells.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta oncologica (Stockholm, Sweden)     Volume:  34     ISSN:  0284-186X     ISO Abbreviation:  Acta Oncol     Publication Date:  1995  
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2009-05-12    
Medline Journal Info:
Nlm Unique ID:  8709065     Medline TA:  Acta Oncol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  213-8     Citation Subset:  IM    
Department of Radiation Oncology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle / drug effects,  radiation effects
Dose-Response Relationship, Radiation
Hela Cells / drug effects,  metabolism,  radiation effects
Immunologic Factors
Radiation-Protective Agents / pharmacology*
Tumor Cells, Cultured / drug effects*,  metabolism,  radiation effects*
Zidovudine / pharmacology*
Reg. No./Substance:
0/Immunologic Factors; 0/Radiation-Protective Agents; 30516-87-1/Zidovudine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Radiation effects on S-phase duration, labelling index, potential doubling time and DNA distribution...
Next Document:  Effects of sucralfate on mucositis during and following radiotherapy of malignancies in the head and...