Document Detail

Azide photochemistry for facile modification of graphitic surfaces: preparation of DNA-coated carbon nanotubes for biosensing.
MedLine Citation:
PMID:  23037575     Owner:  NLM     Status:  MEDLINE    
A facile, two-step method for chemically attaching single-stranded DNA to graphitic surfaces, represented here by carbon nanotubes, is reported. In the first step, an azide-containing compound, N-5-azido-nitrobenzoyloxy succinimide (ANB-NOS), is used to form photo-adducts on the graphitic surfaces in a solid-state photochemical reaction, resulting in active ester groups being oriented for the subsequent reactions. In the second step, pre-synthesized DNA strands bearing a terminal amine group are coupled in an aqueous solution with the active esters on the photo-adducts. The versatility of the method is demonstrated by attaching pre-synthesized DNA to surfaces of carbon nanotubes in two platforms-as vertically-aligned multi-walled carbon nanotubes on a solid support and as tangled single-walled carbon nanotubes in mats. The reaction products at various stages were characterized by x-ray photoelectron spectroscopy. Two different assays were used to check that the DNA strands attached to the carbon nanotubes were able to bind their partner strands with complementary base sequences. The first assay, using partner DNA strands tethered to gold nanoparticles, enabled the sites of DNA attachment to the carbon nanotubes to be identified in TEM images. The second assay, using radioactively labelled partner DNA strands, quantified the density of functional DNA strands attached to the carbon nanotubes. The diversity of potential applications for these DNA-modified carbon-nanotube platforms is exemplified here by the successful use of a DNA-modified single-walled carbon-nanotube mat as an electrode for the specific detection of metal ions.
Minoo J Moghaddam; Wenrong Yang; Barbara Bojarski; Thomas R Gengenbach; Mei Gao; Hadi Zareie; Maxine J McCall
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Publication Detail:
Type:  Journal Article     Date:  2012-10-04
Journal Detail:
Title:  Nanotechnology     Volume:  23     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-05     Completed Date:  2013-08-07     Revised Date:  2013-11-04    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  425503     Citation Subset:  IM    
CSIRO Materials Science and Engineering, North Ryde, NSW 2113, Australia.
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MeSH Terms
Azides / chemistry*
Biosensing Techniques / methods*
DNA / chemistry*
Gold / chemistry
Graphite / chemistry*
Metal Nanoparticles / chemistry,  ultrastructure
Nanotubes, Carbon / chemistry*,  ultrastructure
Phosphorus Radioisotopes
Photochemistry / methods*
Photoelectron Spectroscopy
Surface Properties
Reg. No./Substance:
0/Azides; 0/Nanotubes, Carbon; 0/Phosphorus Radioisotopes; 7440-57-5/Gold; 7782-42-5/Graphite; 9007-49-2/DNA
Erratum In:
Nanotechnology. 2013 Sep 20;24(37):379501

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