Document Detail

The AzgA purine transporter of Aspergillus nidulans. Characterization of a protein belonging to a new phylogenetic cluster.
MedLine Citation:
PMID:  14597637     Owner:  NLM     Status:  MEDLINE    
The azgA gene of Aspergillus nidulans encodes a hypoxanthine-adenine-guanine transporter. It has been cloned by a novel transposon methodology. The null phenotype of azgA was defined by a number of mutations, including a large deletion. In mycelia, the azgA gene is, like other genes of purine catabolism, induced by uric acid and repressed by ammonium. Its transcription depends on the pathway-specific UaY zinc binuclear cluster protein and the broad domain AreA GATA factor. AzgA is not closely related to any other characterized membrane protein, but many close homologues of unknown function are present in fungi, plants, and prokaryotes but not metazoa. Two of three data bases and the phylogeny presented in this article places proteins of this family in a cluster clearly separated (but perhaps phylogenetically related) from the NAT family that includes other eukaryotic and prokaryotic nucleobase transporters. Thus AzgA is the first characterized member of this family or subfamily of membrane proteins.
Gianna Cecchetto; Sotiris Amillis; George Diallinas; Claudio Scazzocchio; Christine Drevet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-11-03
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  279     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-26     Completed Date:  2004-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3132-41     Citation Subset:  IM    
Unidad Asociada de Microbiología, Facultad de Ciencias, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
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MeSH Terms
Adenine / chemistry
Amino Acid Sequence
Aspergillus nidulans / genetics,  metabolism*
Biological Transport
Blotting, Northern
Carrier Proteins / chemistry,  physiology*
Cell Membrane / metabolism
Cloning, Molecular
DNA / chemistry
Escherichia coli / metabolism
Gene Deletion
Hypoxanthine / chemistry,  pharmacokinetics
Membrane Transport Proteins / chemistry*,  physiology
Models, Genetic
Molecular Sequence Data
Multigene Family
Nitrogen / chemistry
Plasmids / metabolism
Protein Structure, Tertiary
Quaternary Ammonium Compounds / pharmacology
Sequence Homology, Amino Acid
Time Factors
Transcription, Genetic
Ultraviolet Rays
Uric Acid / pharmacology
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Transport Proteins; 0/Quaternary Ammonium Compounds; 68-94-0/Hypoxanthine; 69-93-2/Uric Acid; 73-24-5/Adenine; 7727-37-9/Nitrogen; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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