Document Detail


Azetidine-induced accumulation of class I small heat shock proteins in the soluble fraction provides thermotolerance in soybean seedlings.
MedLine Citation:
PMID:  15557296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings to a level similar to that induced by exposure to 40 degrees C. However, only the treatment with 10 mM Aze for 6 h and subsequently with 10 mM proline for 24 h protected the seedlings from damage during subsequent exposure to 45 degrees C as assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. A chaperone activity assay showed that the purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation. Amino acid composition analysis indicated that Aze was not incorporated into de novo synthesized class I sHSPs. Accumulation of class I sHSPs in the soluble post-ribosomal supernatant fraction was found to be important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs and their presence in the soluble fraction are important for establishment of thermotolerance.
Authors:
Tsung-Luo Jinn; Chi-Chou Chiu; Wei-Wen Song; Yih-Ming Chen; Chu-Yung Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-22
Journal Detail:
Title:  Plant & cell physiology     Volume:  45     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-01-17     Completed Date:  2005-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1759-67     Citation Subset:  IM    
Affiliation:
Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan, R.O.C.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence / physiology
Azetidines / metabolism,  pharmacology*
Heat-Shock Proteins / drug effects,  metabolism*
Heat-Shock Response / drug effects,  physiology*
Immunohistochemistry
Microscopy, Electron, Transmission
Molecular Chaperones / drug effects,  metabolism
Plant Roots / drug effects,  metabolism,  ultrastructure
Proline / metabolism,  pharmacology
Protein Denaturation / drug effects,  physiology
Seedling / drug effects,  growth & development,  metabolism*
Soybeans / drug effects,  growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Azetidines; 0/Heat-Shock Proteins; 0/Molecular Chaperones; 147-85-3/Proline; 503-29-7/azetidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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