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Axonal transport of class II and III beta-tubulin: evidence that the slow component wave represents the movement of only a small fraction of the tubulin in mature motor axons.
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MedLine Citation:
PMID:  1383234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pulse-labeling studies demonstrate that tubulin synthesized in the neuron cell body (soma) moves somatofugally within the axon (at a rate of several millimeters per day) as a well-defined wave corresponding to the slow component of axonal transport. A major goal of the present study was to determine what proportion of the tubulin in mature motor axons is transported in this wave. Lumbar motor neurons in 9-wk-old rats were labeled by injecting [35S]methionine into the spinal cord 2 wk after motor axons were injured (axotomized) by crushing the sciatic nerve. Immunoprecipitation with mAbs which recognize either class II or III beta-tubulin were used to analyze the distributions of radioactivity in these isotypes in intact and axotomized motor fibers 5 d after labeling. We found that both isotypes were associated with the slow component wave, and that the leading edge of this wave was enriched in the class III isotype. Axotomy resulted in significant increases in the labeling and transport rates of both isotypes. Immunohistochemical examination of peripheral nerve fibers demonstrated that nearly all of the class II and III beta-tubulin in nerve fibers is located within axons. Although the amounts of radioactivity per millimeter of nerve in class II and III beta-tubulin were significantly greater in axotomized than in control nerves (with increases of +160% and +58%, respectively), immunoassay revealed no differences in the amounts of these isotypes in axotomized and control motor fibers. We consider several explanations for this paradox; these include the possibility that the total tubulin content is relatively insensitive to changes in the amount of tubulin transported in the slow component wave because this wave represents the movement of only a small fraction of the tubulin in these motor fibers.
Authors:
P N Hoffman; M A Lopata; D F Watson; R F Luduena
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  119     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-11-25     Completed Date:  1992-11-25     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  595-604     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287-9204.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Axonal Transport
Axons / physiology*,  ultrastructure
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Male
Microscopy, Immunoelectron
Molecular Sequence Data
Motor Neurons / physiology*,  ultrastructure
Myelin Sheath / physiology,  ultrastructure
Nerve Crush
Nerve Fibers / physiology,  ultrastructure
Neurofilament Proteins / analysis
Oligopeptides / chemical synthesis,  immunology
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins
Sciatic Nerve / physiology*,  ultrastructure
Tubulin / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
NS 22849/NS/NINDS NIH HHS; NS14784/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Neurofilament Proteins; 0/Oligopeptides; 0/Recombinant Fusion Proteins; 0/Tubulin
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Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1992
Volume: 119 Issue: 3
First Page: 595 Last Page: 604
ID: 2289670
Publisher Id: 93016272
PubMed Id: 1383234

Axonal transport of class II and III beta-tubulin: evidence that the slow component wave represents the movement of only a small fraction of the tubulin in mature motor axons


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