Document Detail


Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics.
MedLine Citation:
PMID:  22207757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylcholine (PC) is the most abundant component of lipid bilayers and exists in various molecular forms, through combinations of two acylated fatty acids. Arachidonic acid (AA)-containing PC (AA-PC) can be a source of AA, which is a crucial mediator of synaptic transmission and intracellular signaling. However, the distribution of AA-PC within neurons has not been indicated. In the present study, we used imaging mass spectrometry to characterize the distribution of PC species in cultured neurons of superior cervical ganglia. Intriguingly, PC species exhibited a unique distribution that was dependent on the acyl chains at the sn-2 position. In particular, we found that AA-PC is enriched within the axon and is distributed across a proximal-to-distal gradient. Inhibitors of actin dynamics (cytochalasin D and phallacidin) disrupted this gradient. This is the first report of the gradual distribution of AA-PC along the axon and its association with actin dynamics.
Authors:
Hyun-Jeong Yang; Yuki Sugiura; Koji Ikegami; Yoshiyuki Konishi; Mitsutoshi Setou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-29
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-21     Completed Date:  2012-04-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5290-300     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Hamamatsu 431-3192, Japan.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Arachidonic Acid*
Axons / metabolism*
Biological Transport
Mice
Models, Molecular
Molecular Conformation
Phosphatidylcholines / chemistry*,  metabolism*
Superior Cervical Ganglion / cytology
Chemical
Reg. No./Substance:
0/Actins; 0/Phosphatidylcholines; 506-32-1/Arachidonic Acid
Comments/Corrections

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