Document Detail

Axolotl (Ambystoma mexicanum) lymphocytes produce and are growth-inhibited by transforming growth factor-beta.
MedLine Citation:
PMID:  9617586     Owner:  NLM     Status:  MEDLINE    
Recombinant (r)TGF-beta 5, an isoform of TGF-beta thus far identified only in the frog, Xenopus' laevis, inhibited phytohemagglutinin (PHA)-induced mitogenesis of salamander (axolotl) splenocytes and thymocytes, and T cell growth factor-induced proliferation of splenic lymphoblasts. This inhibition could be reversed by incubating (r)TGF-beta 5 with an anti-TGF-beta 5 antibody, but not with an antibody directed against TGF-beta 2, another Xenopus-produced TGF-beta isoform. Acid-treated supernatants from cultures of PHA-stimulated axolotl splenocytes (PHA-SNs) inhibited proliferation of mink lung fibroblasts (MLF), cells whose growth is sensitive to all known isoforms of TGF-beta. This inhibition was reversed by incubating the supernatants with the anti-pan-specific TGF-beta antibody which neutralizes TGF-beta 1, TGF-beta 2, TGF-beta 3 and TGF-beta 5. This inhibition was unaffected by antibodies that specifically neutralize either TGF-beta 1, TGF-beta 2, TGF-beta 3 or TGF-beta 5. Acid-treated axolotl PHA-SNs also inhibited proliferation of PHA-stimulated freshly harvested axolotl spleen cells. Once again, this inhibition could be reversed by treating supernatants with an anti-pan-specific TGF-beta antibody but not with anti-TGF-beta 2 or anti-TGF-beta 5 antibodies. All these data are consistent with the hypothesis that axolotl lymphocytes secrete a previously unknown TGF-beta isoform.
A Koniski; N Cohen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  22     ISSN:  0145-305X     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:    1998 Jan-Feb
Date Detail:
Created Date:  1998-08-26     Completed Date:  1998-08-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  91-102     Citation Subset:  IM    
Department of Microbiology, University of Rochester Medical Center, NY 14642, USA.
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MeSH Terms
Ambystoma / immunology*
Biological Assay
Lymphocytes / immunology*
Mitogens / analysis
Recombinant Proteins / immunology
Spleen / cytology,  immunology*
Transforming Growth Factor beta / genetics,  immunology*
Grant Support
Reg. No./Substance:
0/Mitogens; 0/Recombinant Proteins; 0/Transforming Growth Factor beta

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