Document Detail


Axl2 integrates polarity establishment, maintenance, and environmental stress response in the filamentous fungus Ashbya gossypii.
MedLine Citation:
PMID:  21984708     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In budding yeast, new sites of polarity are chosen with each cell cycle and polarization is transient. In filamentous fungi, sites of polarity persist for extended periods of growth and new polarity sites can be established while existing sites are maintained. How the polarity establishment machinery functions in these distinct growth forms found in fungi is still not well understood. We have examined the function of Axl2, a transmembrane bud site selection protein discovered in Saccharomyces cerevisiae, in the filamentous fungus Ashbya gossypii. A. gossypii does not divide by budding and instead exhibits persistent highly polarized growth, and multiple axes of polarity coexist in one cell. A. gossypii axl2Δ (Agaxl2Δ) cells have wavy hyphae, bulbous tips, and a high frequency of branch initiations that fail to elongate, indicative of a polarity maintenance defect. Mutant colonies also have significantly lower radial growth and hyphal tip elongation speeds than wild-type colonies, and Agaxl2Δ hyphae have depolarized actin patches. Consistent with a function in polarity, AgAxl2 localizes to hyphal tips, branches, and septin rings. Unlike S. cerevisiae Axl2, AgAxl2 contains a Mid2 homology domain and may function to sense or respond to environmental stress. In support of this idea, hyphae lacking AgAxl2 also display hypersensitivity to heat, osmotic, and cell wall stresses. Axl2 serves to integrate polarity establishment, polarity maintenance, and environmental stress response for optimal polarized growth in A. gossypii.
Authors:
Jonathan F Anker; Amy S Gladfelter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-10-07
Journal Detail:
Title:  Eukaryotic cell     Volume:  10     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-01     Completed Date:  2012-03-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1679-93     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / metabolism
Actins / metabolism
Amino Acid Sequence
Cell Polarity*
Cell Wall / metabolism
Conserved Sequence
Endocytosis
Eremothecium / cytology,  growth & development,  physiology*
Fungal Proteins / genetics,  metabolism,  physiology*
Gene Knockout Techniques
Hyphae / cytology,  growth & development,  physiology*
Membrane Proteins / genetics,  metabolism,  physiology*
Morphogenesis
Protein Structure, Tertiary
Protein Transport
Stress, Physiological*
Chemical
Reg. No./Substance:
0/Actins; 0/Fungal Proteins; 0/Membrane Proteins
Comments/Corrections

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