Document Detail


Availability of Rubisco small subunit up-regulates the transcript levels of large subunit for stoichiometric assembly of its holoenzyme in rice.
MedLine Citation:
PMID:  22811433     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome. For synthesis of the Rubisco holoenzyme, both genes need to be expressed coordinately. To investigate this molecular mechanism, the protein synthesis of two subunits of Rubisco was characterized in transgenic rice (Oryza sativa) plants with overexpression or antisense suppression of the RBCS gene. Total RBCS and rbcL messenger RNA (mRNA) levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants, although the increase in total RBCS mRNA level was greater. In RBCS-antisense plants, the levels of these mRNAs and the synthesis of the corresponding proteins declined to a similar extent. The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes. The level of rbcL mRNA, in turn, was tightly correlated with the amount of RbcL synthesized, the molar ratio of RBCS synthesis to RbcL synthesis being identical irrespective of genotype. Polysome loading of rbcL mRNA was not changed. These results demonstrate that the availability of RBCS protein up-regulates the gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme.
Authors:
Yuji Suzuki; Amane Makino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-17
Journal Detail:
Title:  Plant physiology     Volume:  160     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-04     Completed Date:  2013-01-10     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  533-40     Citation Subset:  IM    
Affiliation:
Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. ysuzuki@biochem.tohoku.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / methods
Chloroplasts / enzymology,  genetics
Enzyme Activation
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant*
Genes, Plant*
Holoenzymes / genetics,  metabolism*
Oryza sativa / enzymology*,  genetics
Photosynthesis
Plant Leaves / enzymology,  genetics
Plant Proteins / genetics
Plants, Genetically Modified / enzymology,  genetics,  metabolism
Polyribosomes / metabolism
Protein Biosynthesis
Protein Subunits / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
RNA, Plant / analysis,  genetics,  metabolism
Ribulose-Bisphosphate Carboxylase / genetics,  metabolism*
Transcription, Genetic
Up-Regulation*
Chemical
Reg. No./Substance:
0/Holoenzymes; 0/Plant Proteins; 0/Protein Subunits; 0/RNA, Messenger; 0/RNA, Plant; EC 4.1.1.39/RbcL protein, plastid; EC 4.1.1.39/Ribulose-Bisphosphate Carboxylase
Comments/Corrections

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