| Autoxidation as a cause of altered lipid distribution in extracts from human red cells. | |
| | |
MedLine Citation:
|
PMID: 5929354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A characteristic alteration in the distribution of human red cell phospholipids represents an artifact due to autoxidation of the lipid extract. This alteration is manifested on silicic acid chromatography by a decrease mainly in the phosphatidyl ethanolamine and phosphatidyl serine fractions (probably because of their abundance of highly unsaturated fatty acids) and an increase in the phospholipid recovered with the more polar fractions, sphingomyelin and lysolecithin. No evidence was found for "lysocephalin" formation or plasmalogen breakdown in dry lipid extracts after autoxidation by exposure to air at room temperature for 24-35 hr. On thin-layer chromatography, however, the ninhydrin-positive streaking in the autoxidized samples may be erroneously attributed to the presence of "lyso" derivatives. When the alterations in lipid distribution described above are found, the possibility of this artifact should be considered. |
| | |
Authors:
|
J T Dodge; G B Phillips |
Related Documents
:
|
3676294 - Deuterium nuclear magnetic resonance studies on the plasmalogens and the glycerol aceta... 11970734 - Hydrocarbons, fatty acids, and lipids of freshwater grasses of the potamogetonaceae fam... 2247434 - Stability of fatty acids and leaf protein concentrate of persian clover (trifolium resu... 5378384 - The isolation and characterization of trigalactosyl diglyceride from potato tubers. 2291464 - High-performance liquid chromatography of sialic acid-containing oligosaccharides and a... 3583754 - Radiometric studies on the oxidation of [1-14c]fatty acids and [u-14c]l-amino acids by ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of lipid research Volume: 7 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1966 May |
Date Detail:
|
Created Date: 1967-02-28 Completed Date: 1967-02-28 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 387-95 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Gas Chromatography, Thin Layer Erythrocytes / analysis, metabolism* Fatty Acids Humans Lysophosphatidylcholines / analysis Oxidation-Reduction* Phosphatidylethanolamines / analysis Phospholipids / metabolism* Spectrum Analysis Sphingomyelins / analysis Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Sphingomyelins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cerebroside galactosidase of brain.
Next Document: Lipid composition of rat mammary carcinomas, mammary glands, and related tissues: endocrine influenc...