Document Detail


Autoradiography reveals regional metabolic differences in the endometrium of pregnant and nonpregnant mice.
MedLine Citation:
PMID:  9686154     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rodent endometrium undergoes remarkable modifications during pregnancy, resulting from a redifferentiation of its fibroblasts. During this modification (decidualization), the fibroblasts transform into large, polyhedral cells that establish intercellular junctions. Decidualization proceeds from the subepithelial stroma towards the deep stroma situated next to the myometrium and creates regions composed of cells in different stages of differentiation. We studied by autoradiography whether cells of these different regions have different levels of macromolecular synthesis. Radioactive amino acids or radioactive sulfate were administered to mice during estrus or on different days of pregnancy. The animals were killed 30 min after injection of the precursors and the uteri were processed for light microscope autoradiography. Silver grains were counted over cells of different regions of the endometrium and are reported as the number of silver grains per area. Higher levels of incorporation of amino acids were found in pregnant animals as compared to animals in estrus. In pregnant animals, the region of decidual cells or the region of fibroblasts transforming into decidual cells showed the highest levels of synthesis. Radioactive sulfate incorporation, on the other hand, was generally higher in nonpregnant animals. Animals without decidual cell transformation (nonpregnant and 4th day of pregnancy) showed a differential incorporation by subepithelial and deep stroma fibroblasts. This study shows that regional differences in synthetic activity exist in cells that are in different stages of transformation into decidual cells as well as in different regions of the endometrium of nonpregnant mice.
Authors:
S F Oliveira; P Abrahamsohn; T M Zorn
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  31     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-10-22     Completed Date:  1998-10-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  307-12     Citation Subset:  IM    
Affiliation:
Departamento de Histologia e Embriologia, Universidade de São Paulo, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography*
Cell Differentiation / physiology
Decidua / cytology,  metabolism*
Embryo Implantation
Endometrium / cytology,  metabolism*
Female
Fibroblasts / cytology
Heparinoids / pharmacokinetics
Mice
Pregnancy / metabolism*
Proline / pharmacokinetics
Stromal Cells / cytology
Tryptophan / pharmacokinetics
Chemical
Reg. No./Substance:
0/Heparinoids; 147-85-3/Proline; 73-22-3/Tryptophan

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