| Autoradiography of live and dead mammalian cells with 99mTc-tetracycline. | |
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MedLine Citation:
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PMID: 1113188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Considering the diagnostic significance of a few 99mTc-chelates in the diagnosis of myocardial infarct, we have recently developed a necrosis model in tissue culture obtained from mammalian cells, where the dead cells showed enhanced binding of 99mTc-tetracycline like the necrotic cells of infarcted myocardium. Technical details of the principles of labeling, fixing, washing and drying, dipping in emulsion, exposure, development and staining of mammalian cells have been described. Considering the short half-life of 99mTc-radioisotope, the recently available techniques have been modified to make autoradiography possible in a reasonably short period of time. Technetium-99m in different physical and chemical states is playing a very important role in a variety of diagnostic procedures. The autoradiography of 99mTc-labeled cells and tissue will shed further light on the mechanism of cellular uptake and subcellular distribution in normal and pathologic states. Presently no information is available regarding autoradiography with 99mTc-chelates of mammalian cells obtained from tissue culture. |
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Authors:
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M K Dewanjee |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of nuclear medicine : official publication, Society of Nuclear Medicine Volume: 16 ISSN: 0161-5505 ISO Abbreviation: J. Nucl. Med. Publication Date: 1975 Apr |
Date Detail:
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Created Date: 1975-05-21 Completed Date: 1975-05-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217410 Medline TA: J Nucl Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 315-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autoradiography
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methods* Myocardial Infarction / diagnosis Necrosis / diagnosis* Technetium* Tetracycline / diagnostic use* |
| Chemical | |
Reg. No./Substance:
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60-54-8/Tetracycline; 7440-26-8/Technetium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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