Document Detail


Autoradiography of 32P and 14C incorporation into protoplasts as a means of determining the percentage of virus infected protoplasts.
MedLine Citation:
PMID:  6885954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stripping Film Autoradiography (SFA) was used to measure percentage protoplast infection. Control and infected protoplasts were incubated in the presence of [32P]orthophosphate or [14C]leucine fixed onto glass slides and placed in contact with a sensitive photographic emulsion. Infected protoplasts were distinguished by blackened areas of exposed film which coincided with protoplasts containing labelled virus products. The use of SFA is demonstrated for two different virus/protoplast systems, namely tobacco mosaic virus (TMV) in tobacco and turnip yellow mosaic virus (TYMV) in rape. The method detects infection as early as 12-16 h post-inoculation and UV irradiation is not necessary for reducing background interference from labelled host proteins.
Authors:
F M Cocker; A C Cassells
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virological methods     Volume:  6     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-10-21     Completed Date:  1983-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  311-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autoradiography / methods*
Carbon Radioisotopes
Mosaic Viruses / analysis*,  metabolism
Phosphorus Radioisotopes
Plant Diseases*
Time Factors
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Phosphorus Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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