Document Detail


Autoradiographic localization and effects of exogenous radioactively labelled surfactant in the lung of the prematurely delivered fetal rabbit.
MedLine Citation:
PMID:  1790253     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult rabbit lung surfactant was radioactively labelled with [3H]palmitate and isolated by centrifugation. This material was instilled into the trachea of fetal rabbits prematurely delivered on the 27th gestational day. A similar preparation of unlabelled surfactant was used to measure the effects on pressure-volume characteristics in lungs of 27th day fetuses. Tissue sections were prepared from the lungs of all animals and morphometric and autoradiographic determinations made. Surfactant instillation improved pressure-volume relationships in fetal rabbit lungs. Histologically, although only the middle right lobe seemed to show significant qualitative improvement in expansion after surfactant treatment, quantitative assessment indicated that the surfactant preparation had significantly increased the mean alveolar cross-sectional areas in all three lobes of right lungs. In addition, distribution of autoradiographic grains indicated that 8-25% were located over the alveolar spaces while approximately half this percentage was present over tissue at the level of the alveolus. These results indicate that intratracheal instillation of surfactant supplements the endogenous surfactant at the level of the alveolus.
Authors:
I Winter; J E Scott; M R Oulton; D Stinson; A Allen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the neonate     Volume:  60     ISSN:  0006-3126     ISO Abbreviation:  Biol. Neonate     Publication Date:  1991  
Date Detail:
Created Date:  1992-03-30     Completed Date:  1992-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0247551     Medline TA:  Biol Neonate     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  292-302     Citation Subset:  IM    
Affiliation:
Department of Oral Biology, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn*
Autoradiography
Embryonic and Fetal Development / drug effects*
Female
Lung / chemistry*,  drug effects
Palmitates / diagnostic use*
Phospholipids / analysis
Pregnancy
Pulmonary Alveoli / drug effects
Pulmonary Surfactants / diagnostic use*,  pharmacology
Rabbits
Tritium
Chemical
Reg. No./Substance:
0/Palmitates; 0/Phospholipids; 0/Pulmonary Surfactants; 10028-17-8/Tritium

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