Document Detail

Autoradiographic localization of atrial and brain natriuretic peptide receptors in rat brain.
MedLine Citation:
PMID:  2154136     Owner:  NLM     Status:  MEDLINE    
Displacement of bound 125I-labeled atrial natriuretic peptide-(1-28) [alpha 125I-ANP-(1-28)] by alpha-ANP-(5-28) and porcine brain natriuretic peptide (BNP) was used to map receptors common to these peptides in rat brain by in vitro autoradiography. alpha-125I-ANP bound reversibly to subfornical organ, area postrema, median preoptic, supraoptic and paraventricular nuclei, and arachnoid mater. Binding at these sites was displaced similarly by 1 microM unlabeled alpha-ANP, alpha-ANP-(5-28), and BNP. Binding dissociation constants in the subfornical organ and arachnoid were 4.40 +/- 1.15 and 3.99 +/- 0.86 nM, respectively, for alpha-ANP, and 2.41 +/- 1.11 and 2.23 +/- 1.06 nM, respectively, for BNP. alpha-125I-ANP also bound to choroid plexus. Here 1 microM unlabeled alpha-ANP displaced significantly more radioligand than did 1 microM BNP, and the concentration displacing 50% of bound radioligand was 2.23 +/- 0.78 nM for alpha-ANP and 1.51 +/- 0.67 nM for BNP. alpha-ANP-(5-28) also displaced alpha 125I-ANP at all sites with significantly greater affinity than did unlabeled alpha-ANP. alpha-125I-ANP was not displaced by completely unrelated peptides. Therefore, both atrial and brain natriuretic peptides may be high-affinity ligands at common receptors in some cerebral localities.
J Brown; A Czarnecki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  258     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-05     Completed Date:  1990-03-05     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R57-63     Citation Subset:  IM    
Physiological Laboratory, Cambridge, United Kingdom.
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MeSH Terms
Atrial Natriuretic Factor / metabolism
Binding, Competitive
Brain / metabolism*
Nerve Tissue Proteins / metabolism
Peptide Fragments / metabolism
Rats, Inbred Strains
Receptors, Atrial Natriuretic Factor
Receptors, Cell Surface / metabolism*
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Peptide Fragments; 0/Receptors, Cell Surface; 0/brain natriuretic peptide receptor; 85637-73-6/Atrial Natriuretic Factor; 98897-20-2/atrial natriuretic factor (5-28); EC, Atrial Natriuretic Factor

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