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Autophagy suppresses IL-1β signaling via the regulation of p62 stability.
MedLine Citation:
PMID:  22167182     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ATG16L1 is an essential component of the autophagasome. The T300A allele of ATG16L1 is associated with the increased susceptibility to Crohns disease. In this study, we identified a novel function of ATG16L1, which suppresses signaling of the pro-inflammatory cytokine IL-1b. Deletion of ATG16L1 in mouse embryonic fibroblasts significantly amplifies IL-1b signal transduction cascades. This amplification is due to elevated p62 levels in ATG16L1-deficient cells. We found that ATG16L1 regulates p62 levels via both autolysosomal and proteasomal pathways. For proteasomal degradation, we found that Cullin-3 (Cul-3) is a E3 ubiquitin ligase of p62 and that ATG16L1 is essential for neddylation of Cul-3, a step required for Cul-3 activation. Taken together our data indicate that loss-of-function of ATG16L1 in fibroblasts results in a hyper-responsiveness to the IL-1b due to the increased p62 level.
Authors:
Jongdae Lee; Hyeri Kim; Christine Quinley; Joanna Kim; Jose Gonzalez-Navajas; Ramnik Xavier; Eyal Raz
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-13
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  -     ISSN:  1083-351X     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of California, San Diego, United States;
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