Document Detail

Autophagy is not universally required for phosphatidyl-serine exposure and apoptotic cell engulfment during neural development.
MedLine Citation:
PMID:  19587526     Owner:  NLM     Status:  MEDLINE    
Apoptosis and autophagy are physiological processes implicated in the maintenance of cell and tissue homeostasis. We took advantage of the existence of multiple phases of developmental cell death in the embryonic chick retina and of the availability of short-term organotypic retinal cultures to explore the possible relationship between apoptosis and autophagy during neural development. We examined retinas at embryonic day 5, an early stage at which cell death is related to eye morphogenesis and to retinal ganglion cell generation, as well as at embryonic day 9, when cell death is associated with neurotrophic support of the retinal ganglion cells. Exposure to 3-methyl-adenine, a classical inhibitor of autophagy, elicited a selective accumulation of apoptotic bodies in the dorsotemporal area of embryonic day 5 retinas where neurogenesis is taking place. This accumulation was correlated with a blockage of phosphatidyl-serine presentation and, consequently, with a lack of engulfment of the dying cells by their neighbors. In striking contrast, none of these phenomena were observed in association with cell death in the optic nerve and optic fissure at embryonic day 5, or in embryonic day 9 retinas. Our data suggest that autophagy is essential for phosphatidyl-serine presentation by apoptotic cells during the phase of cell death associated to neurogenesis, but this is not a universal requirement for all phases of cell death occurring during retinal development.
Mar??a A Mell??n; Enrique J de la Rosa; Patricia Boya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-18
Journal Detail:
Title:  Autophagy     Volume:  5     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-19     Completed Date:  2010-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  964-72     Citation Subset:  IM    
3D Lab (Development, Differentiation & Degeneration), Cellular and Molecular Medicine, Centro de Investigaciones Biol??gicas, CSIC, Madrid, Spain.
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MeSH Terms
Apoptosis / physiology*
Autophagy / physiology*
Avian Proteins / genetics,  metabolism
Chick Embryo
In Situ Nick-End Labeling
Membrane Glycoproteins / genetics,  metabolism
Neurogenesis / physiology*
Neurons* / cytology,  physiology
Phosphatidylserines / metabolism*
Retina / cytology,  embryology,  metabolism
Reg. No./Substance:
0/Avian Proteins; 0/Membrane Glycoproteins; 0/Phosphatidylserines; 132965-98-1/LEP100 protein, Gallus gallus

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