Document Detail


Autophagy and leucine promote chronological longevity and respiration proficiency during calorie restriction in yeast.
MedLine Citation:
PMID:  23337777     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We have previously shown that autophagy is required for chronological longevity in the budding yeast Saccharomyces cerevisiae. Here we examine the requirements for autophagy during extension of chronological life span (CLS) by calorie restriction (CR). We find that autophagy is upregulated by two CR interventions that extend CLS: water wash CR and low glucose CR. Autophagy is required for full extension of CLS during water wash CR under all growth conditions tested. In contrast, autophagy was not uniformly required for full extension of CLS during low glucose CR, depending on the atg allele and strain genetic background. Leucine status influenced CLS during CR. Eliminating the leucine requirement in yeast strains or adding supplemental leucine to growth media extended CLS during CR. In addition, we observed that both water wash and low glucose CR promote mitochondrial respiration proficiency during aging of autophagy-deficient yeast. In general, the extension of CLS by water wash or low glucose CR was inversely related to respiration deficiency in autophagy-deficient cells. Also, autophagy is required for full extension of CLS under non-CR conditions in buffered media, suggesting that extension of CLS during CR is not solely due to reduced medium acidity. Thus, our findings show that autophagy is: (1) induced by CR, (2) required for full extension of CLS by CR in most cases (depending on atg allele, strain, and leucine availability) and, (3) promotes mitochondrial respiration proficiency during aging under CR conditions.
Authors:
John P Aris; Ashley L Alvers; Roy A Ferraiuolo; Laura K Fishwick; Amanda Hanvivatpong; Doreen Hu; Christine Kirlew; Michael T Leonard; Kyle J Losin; Michelle Marraffini; Arnold Y Seo; Veronica Swanberg; Jennifer L Westcott; Michael S Wood; Christiaan Leeuwenburgh; William A Dunn
Related Documents :
14587947 - Light-activated antimicrobial coating for the continuous disinfection of surfaces.
18551717 - Confinement effects on the morphology of photopatterned porous polymer monoliths for ca...
9663737 - Morphological behaviour of osteoblasts on diamond-like carbon coating and amorphous c-n...
18726187 - Corrosion resistance evaluation of a ca- and p-based bioceramic thin coating in ti-6al-4v.
19665847 - Loop-mediated isothermal amplification (lamp) detection of babesia orientalis in water ...
24287967 - Large pore mesostructured cellular silica foam coated magnetic oxide composites with mu...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-18
Journal Detail:
Title:  Experimental gerontology     Volume:  -     ISSN:  1873-6815     ISO Abbreviation:  Exp. Gerontol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0047061     Medline TA:  Exp Gerontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Department of Anatomy and Cell Biology, University of Florida, Health Science Center, 1600 SW Archer Road, Gainesville, FL 32610-0235. Electronic address: johnaris@ufl.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Soft templating strategies for the synthesis of mesoporous materials: Inorganic, organic-inorganic h...
Next Document:  Characteristics of cardiac aging in C57BL/6 mice.