| Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation. | |
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MedLine Citation:
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PMID: 19778961 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Autophagy is triggered when organisms sense radical environmental changes, including nutritional starvation. During autophagy, cytoplasmic components, including organelles, are enclosed within autophagosomes and are degraded upon lysosome-vacuole fusion. In this study, we show that processing of GFP-tagged Atg8 can serve as a marker for autophagy in the fission yeast Schizosaccharomyces pombe. Using this marker, 13 Atg homologues were also found to be required for autophagy in fission yeast. In budding yeast, autophagy-deficient mutants are known to be sterile, whereas in fission yeast we found that up to 30 % of autophagy-defective cells with amino acid auxotrophy were able to recover sporulation when an excess of required amino acids was supplied. Furthermore, we found that approximately 15 % of the autophagy-defective cells were also able to sporulate when a prototrophic strain was subjected to nitrogen starvation, which suggested that fission yeast may store sufficient intracellular nitrogen to allow partial sporulation under nitrogen-limiting conditions, although the majority of the nitrogen source is supplied by autophagy. Monitoring of the sporulation process revealed that the process was blocked non-specifically at various stages in the atg1Delta and atg12Delta mutants, possibly due to a shortage of amino acids. Taking advantage of this partial sporulation ability of fission yeast, we sought evidence for the existence of a recycling system for nitrogen sources during starvation. |
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Authors:
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Hiroyuki Mukaiyama; Shiro Kajiwara; Akira Hosomi; Yuko Giga-Hama; Naotaka Tanaka; Taro Nakamura; Kaoru Takegawa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-24 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 155 ISSN: 1465-2080 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
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Languages: eng Pagination: 3816-26 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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