Document Detail


Autophagy is active in normal colon mucosa.
MedLine Citation:
PMID:  22652752     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, autophagy has been found to be strongly activated in colon cancer cells, but few studies have addressed the normal colon mucosa. The aim of this study was to characterize autophagy in normal human intestinal cells. We used the expression of LC3-II and BECN1 as well as SQSTM1 as markers of autophagy activity. Using the normal human intestinal epithelial crypt (HIEC) cell experimental model, we found that autophagy was much more active in undifferentiated cells than in differentiated cells. In the normal adult colonic mucosa, BECN1 was found in the proliferative epithelial cells of the lower part of the gland while SQSTM1 was predominantly found in the differentiated cells of the upper part of the gland and surface epithelium. Interestingly, the weak punctate pattern of SQSTM1 expression in the lower gland colocalized with BECN1-labeled autophagosomes. The usefulness of SQSTM1 as an active autophagy marker was confirmed in colon cancer specimens at the protein and transcript levels. In conclusion, our results show that autophagy is active in the colonic gland and is associated with the intestinal proliferative/undifferentiated and progenitor cell populations.
Authors:
Jean-Francois Groulx; Taoufik Khalfaoui; Yannick D Benoit; Gérald Bernatchez; Julie C Carrier; Nuria Basora; Jean-François Beaulieu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-01
Journal Detail:
Title:  Autophagy     Volume:  8     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-08-24     Completed Date:  2013-01-03     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  893-902     Citation Subset:  IM    
Affiliation:
CIHR Team on the Digestive Epithelium, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec Canada.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / metabolism
Adult
Aged
Aged, 80 and over
Apoptosis Regulatory Proteins / metabolism
Autophagy*
Biological Markers / metabolism
Cell Differentiation
Cell Line, Tumor
Colon / cytology*,  metabolism
Colorectal Neoplasms / metabolism,  pathology
Down-Regulation
Enterocytes / cytology,  metabolism
Fluorescent Antibody Technique
Humans
Intestinal Mucosa / cytology*,  metabolism
Membrane Proteins / metabolism
Middle Aged
Models, Biological
Grant Support
ID/Acronym/Agency:
MOP-57727//Canadian Institutes of Health Research; MOP-97836//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Apoptosis Regulatory Proteins; 0/BECN1 protein, human; 0/Biological Markers; 0/Membrane Proteins; 0/SQSTM1 protein, human
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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