| Autophagy is activated, but is not required for the G0 function of BCL-2 or BCL-xL. | |
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MedLine Citation:
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PMID: 18758240 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle arrest in G(0) and autophagy have features in common, but the inter-relationship between the two processes is not well defined. The anti-apoptosis molecules BCL-2 and BCL-x(L) promote G(0) arrest through upregulation of p27 protein, which can also induce autophagy. We tested the hypothesis that autophagy was involved in the cell cycle arrest function of BCL-2 and BCL-x(L). We found that in IL-3-dependent FL5.12 cells, NIH3T3 cells and mouse embryo fibroblasts induced to arrest, treatment with 3-methyladenine did not affect the expected decrease in cell size and ribosomal RNA synthesis, or upregulation of p27 levels. Using the m5-7 ATG5(-/-) MEF cell line with doxycycline-regulated ATG5 expression, we demonstrated that autophagy was activated during serum withdrawal and contact inhibition, but inhibition of autophagy had no measurable effect on G(0) arrest in parental or BCL-x(L)-expressing cells. Thus, our data indicate that, in cell culture models, autophagy occurs but is not required for entrance into quiescence or for the G(0) function of BCL-2 or BCL-x(L). |
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Authors:
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Mayda Valentin; Elizabeth Yang |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-09-12 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 7 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-04 Completed Date: 2008-10-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 2762-8 Citation Subset: IM |
Affiliation:
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Departments of Cancer Biology and Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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analogs & derivatives,
pharmacology Animals Autophagy* / drug effects CHO Cells Cell Size / drug effects Cricetinae Cricetulus Cyclin-Dependent Kinase Inhibitor p27 / metabolism Fibroblasts / cytology*, drug effects G0 Phase* / drug effects Humans Mice Microtubule-Associated Proteins / metabolism NIH 3T3 Cells RNA / metabolism Up-Regulation / drug effects bcl-X Protein / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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2R01CA78443/CA/NCI NIH HHS; 5T32CA009384/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Atg5 protein, mouse; 0/Microtubule-Associated Proteins; 0/bcl-X Protein; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 5142-23-4/3-methyladenine; 63231-63-0/RNA; 73-24-5/Adenine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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