| Autophagic cell death is induced by acetone and ethyl acetate extracts from Eupatorium odoratum in vitro: effects on MCF-7 and vero cell lines. | |
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MedLine Citation:
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PMID: 22666123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity, membrane blebbing and ballooning at 24 hours, replacement by mass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts of EO induce cell death through induction of autophagy and hold potential for development as potential anticancer drugs. |
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Authors:
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Faizah Bt Harun; Syed Mohsin Syed Sahil Jamalullail; Khoo Boon Yin; Zulkhairi Othman; Anita Tilwari; Prabha Balaram |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2012-04-30 |
Journal Detail:
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Title: TheScientificWorldJournal Volume: 2012 ISSN: 1537-744X ISO Abbreviation: ScientificWorldJournal Publication Date: 2012 |
Date Detail:
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Created Date: 2012-06-05 Completed Date: 2012-10-26 Revised Date: 2013-02-28 |
Medline Journal Info:
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Nlm Unique ID: 101131163 Medline TA: ScientificWorldJournal Country: England |
Other Details:
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Languages: eng Pagination: 439479 Citation Subset: IM |
Affiliation:
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Institute for Research in Molecular Medicine-INFORMM, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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chemistry Acetone / chemistry Animals Autophagy / drug effects* Cell Death / drug effects* Cell Line, Tumor Cercopithecus aethiops Chromolaena / chemistry* Humans Plant Extracts / pharmacology* Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Plant Extracts; 67-64-1/Acetone; 76845O8NMZ/ethyl acetate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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