Document Detail


Autonomous replication in vivo and in vitro of clones spanning the region of the DHFR origin of bidirectional replication (ori beta).
MedLine Citation:
PMID:  7828889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasmids containing the origin of bidirectional replication (ori beta) of the Chinese hamster dihydrofolate reductase-encoding gene (DHFR) were tested for autonomous replication in vivo and in vitro. The results show that plasmids pX24 and pneoS13, that contain a 4.8- and a 11.5-kb fragment, respectively, spanning the ori beta region, are able to replicate autonomously in human cells and in a cell-free system that uses human cell extracts. Another plasmid, pX14, containing a 4.8-kb fragment that is immediately adjacent to the ori beta region, also replicated in these two assays.
Authors:
M Zannis-Hadjopoulos; T O Nielsen; A Todd; G B Price
Related Documents :
9326669 - Long-term potentiation activates the gap-43 promoter: selective participation of hippoc...
11017879 - Inhibition of mmh (met murine hepatocyte) cell differentiation by tgf(beta) is abrogate...
10194529 - Beta-cell gene expression and functional characterisation of the human insulinoma cell ...
18843049 - Regulation of the anaphase-promoting complex-separase cascade by transforming growth fa...
19939939 - Conditional mutation of pkd2 causes cystogenesis and upregulates beta-catenin.
10459019 - Exogenous expression of beta-catenin regulates contact inhibition, anchorage-independen...
17703109 - Extracellular matrix induces doxorubicin-resistance in human osteosarcoma cells by supp...
16421249 - Rgf1p is a specific rho1-gef that coordinates cell polarization with cell wall biogenes...
17270179 - Three-dimensional cell organization leads to almost immediate hre activity as demonstra...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  151     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-02-22     Completed Date:  1995-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  273-7     Citation Subset:  IM    
Affiliation:
McGill Cancer Centre, McGill University, Montréal, Québec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cloning, Molecular / methods
Cricetinae
Cricetulus
DNA Replication*
Hela Cells
Humans
Plasmids
Polymerase Chain Reaction
Replication Origin*
Restriction Mapping
Templates, Genetic
Tetrahydrofolate Dehydrogenase / genetics*
Transfection
Chemical
Reg. No./Substance:
EC 1.5.1.3/Tetrahydrofolate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Alternative splicing of the mRNA encoding baboon glycoprotein receptor GPIIb.
Next Document:  Cloning and analysis of human cDNAs encoding a 140-kDa brain guanine nucleotide-exchange factor, Cdc...