Document Detail


Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells.
MedLine Citation:
PMID:  17615405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mice, unique events regulating epigenetic programming (e.g., genomic imprinting) and replication state (mitosis versus meiosis) occur during fetal germ cell development. To determine whether these processes are autonomously programmed in fetal germ cells or are dependent upon ongoing instructive interactions with surrounding gonadal somatic cells, we isolated male and female germ cells at 13.5 days postcoitum (dpc) and maintained them in culture for 6 days, either alone or in the presence of feeder cells or gonadal somatic cells. We examined allele-specific DNA methylation in the imprinted H19 and Snrpn genes, and we also determined whether these cells remained mitotic or entered meiosis. Our results show that isolated male germ cells are able to establish a characteristic "paternal" methylation pattern at imprinted genes in the absence of any support from somatic cells. On the other hand, cultured female germ cells maintain a hypomethylated status at these loci, characteristic of the normal "maternal" methylation pattern in endogenous female germ cells before birth. Further, the surviving female germ cells entered first meiotic prophase and reached the pachytene stage, whereas male germ cells entered mitotic arrest. These results indicate that mechanisms controlling both epigenetic programming and replication state are autonomously regulated in fetal germ cells that have been exposed to the genital ridge prior to 13.5 dpc.
Authors:
Kazuhiro Iwahashi; Hirotaka Yoshioka; Eleanor W Low; John R McCarrey; Ryuzo Yanagimachi; Yukiko Yamazaki
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-07-05
Journal Detail:
Title:  Biology of reproduction     Volume:  77     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-21     Completed Date:  2007-11-13     Revised Date:  2011-10-07    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  697-706     Citation Subset:  IM    
Affiliation:
Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96813, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Autoantigens / genetics
Cells, Cultured
DNA (Cytosine-5-)-Methyltransferase / metabolism
DNA Methylation
Female
Fetus / cytology*,  metabolism
Gene Expression Regulation, Developmental*
Genomic Imprinting*
Germ Cells / cytology,  growth & development*,  metabolism
Gonads / cytology,  embryology
Male
Mice
Mice, Transgenic
RNA, Untranslated / genetics
Ribonucleoproteins, Small Nuclear / genetics
Sex Differentiation / genetics*
Sexual Development / genetics*
snRNP Core Proteins
Grant Support
ID/Acronym/Agency:
HD042772/HD/NICHD NIH HHS; P20RR16467/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Autoantigens; 0/H19 long non-coding RNA; 0/RNA, Untranslated; 0/Ribonucleoproteins, Small Nuclear; 0/snRNP Core Proteins; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase

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