Document Detail

Autonomous and non-autonomous Shh signalling mediate the in vivo growth and guidance of mouse retinal ganglion cell axons.
MedLine Citation:
PMID:  18832395     Owner:  NLM     Status:  MEDLINE    
In non-mammalian vertebrates, the relatively homogeneous population of retinal ganglion cells (RGCs) differentiates and projects entirely to the contralateral side of the brain under the influence of sonic hedgehog (Shh). In mammals, by contrast, there are two different RGC types: the Zic2-positive ipsilateral projecting and the Isl2-positive contralateral projecting. We asked whether the axons of these two populations respond to Shh and if their response differs. We have also analysed whether midline- and RGC-derived Shh contributes to the growth of the axons in the proximal visual pathway. We show that these two RGC types are characterised by a differential expression of Shh signalling components and that they respond differently to Shh when challenged in vitro. In vivo blockade of Shh activity, however, alters the path and distribution mostly of the contralateral projecting RGC axons at the chiasm, indicating that midline-derived Shh participates in funnelling contralateral visual fibres in this region. Furthermore, interference with Shh signalling in the RGCs themselves causes abnormal growth and navigation of contralateral projecting axons in the proximal portion of the pathway, highlighting a novel cell-autonomous mechanism by which Shh can influence growth cone behaviour.
Cristina Sánchez-Camacho; Paola Bovolenta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-02
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  135     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2008-12-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3531-41     Citation Subset:  IM    
Departamento de Neurobiología Molecular Celular y del Desarrollo, Instituto Cajal, CSIC and CIBER de Enfermedades Raras (CIBERER) 37, Madrid 28002, Spain.
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MeSH Terms
Axons / metabolism*
Embryo, Mammalian / cytology,  metabolism
Gene Expression Regulation, Developmental
Growth Cones / metabolism
Hedgehog Proteins / genetics,  metabolism*
Mice, Inbred C57BL
Neurites / metabolism
Optic Chiasm / cytology,  metabolism
Receptors, Cell Surface / metabolism
Retinal Ganglion Cells / cytology*,  metabolism*
Signal Transduction*
Transcription, Genetic
Visual Pathways / cytology,  metabolism
Reg. No./Substance:
0/Hedgehog Proteins; 0/Receptors, Cell Surface; 0/Shh protein, mouse; 0/patched receptors

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