| Automation of metabolic stability studies in microsomes, cytosol and plasma using a 215 Gilson liquid handler. | |
| | |
MedLine Citation:
|
PMID: 10698555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A 215 Gilson liquid handler was used to automate enzymatic incubations using microsomes, cytosol and plasma. The design of automated protocols are described. They were based on the use of 96 deep well plates and on HPLC-based methods for assaying the substrate. The assessment of those protocols was made with comparison between manual and automated incubations, reliability and reproducibility of automated incubations in microsomes and cytosol. Examples of the use of those programs in metabolic studies in drug research, i.e. metabolic screening in microsomes and plasma were shown. Even rapid processes (with disappearance half lives as low as 1 min) can be analysed. This work demonstrates how stability studies can be automated to save time, render experiments involving human biological media less hazardous and may be improve inter-laboratory reproducibility. |
| | |
Authors:
|
J M Linget; P du Vignaud |
Related Documents
:
|
6091495 - A fluorescent screening assay for collagenase using collagen labeled with 2-methoxy-2,4... 1689165 - A new sensitive bioassay for precise quantification of interferon activity as measured ... 6638505 - A microfluorometric assay for cholinesterases, suitable for multiple kinetic determinat... 21733405 - In situ intestinal perfusion of irinotecan: application to p-gp mediated drug interacti... 18374345 - Applicability of ultra-performance liquid chromatography-tandem mass spectrometry for h... 12401235 - Application of the novel fluorescent dye beljian red to the differentiation of giardia ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of pharmaceutical and biomedical analysis Volume: 19 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1999 May |
Date Detail:
|
Created Date: 2000-03-21 Completed Date: 2000-03-21 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 893-901 Citation Subset: IM |
Affiliation:
|
Laboratoire de recherche Glaxo Wellcome France, Les Ulis. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetonitriles
/
chemistry Animals Automation Chromatography, High Pressure Liquid / methods Computer-Aided Design Cytosol / metabolism Female Humans Male Microsomes / metabolism* Oxazines / analysis Plasma / metabolism Rats Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
|
0/Acetonitriles; 0/Oxazines; 635-78-9/resorufin; 75-05-8/acetonitrile |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Kinase receptor activation (KIRA): a rapid and accurate alternative to end-point bioassays.
Next Document: Determination of a novel bile acid sequestrant in rodent diet by near-infrared spectroscopy.