Document Detail

Automatic dendritic spine analysis in two-photon laser scanning microscopy images.
MedLine Citation:
PMID:  17654649     Owner:  NLM     Status:  MEDLINE    
Dendritic spine expression plays an important role in the central nervous system. Modern fluorescence microscopy and green fluorescent protein technology have facilitated the research on spines. To quantitatively analyze the spines in fluorescence microscopy images, an automatic dendritic spine analysis method is proposed. Because of the limit of axial resolution, our method is designed to process the projection image along the z-axis and analyze the lateral spines. The method can automatically extract the dendrite centerlines and segment the spines along the dendrites according to width-based criteria. The criteria utilize a common morphological feature of the spines. It can detect some shapes of spines missed by previous methods. In addition, the proposed method is automatic once a few parameters are set. Spine numbers, lengths, and densities, which biologists are interested in, are analyzed both manually and automatically. The results of the two methods match well. The proposed method provides automatic and accurate dendritic spine analysis. It can serve as a useful tool for spine image analysis to avoid tedious manual labor.
Wenjia Bai; Xiaobo Zhou; Liang Ji; Jie Cheng; Stephen T C Wong
Related Documents :
12079219 - Photodynamic therapy and fluorescence diagnosis of skin cancers.
8705599 - Light source-induced error in computer-assisted image analysis with a video-based system.
18542519 - Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat br...
22942389 - Rosette tracker: an open source image analysis tool for automatic quantification of gen...
18552949 - Fast handheld two-photon fluorescence microendoscope with a 475 microm x 475 microm fie...
1341249 - Detection of squamous cell cancer and pre-cancerous lesions by imaging of tissue autofl...
20451879 - Addressing population aging and alzheimer's disease through the australian imaging biom...
19910159 - Effect of acoustic power on in vivo molecular imaging with targeted microbubbles: impli...
22388629 - Radiolabelled rgd peptides for imaging and therapy.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  71     ISSN:  1552-4922     ISO Abbreviation:  Cytometry A     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-26     Completed Date:  2007-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  818-26     Citation Subset:  IM    
Bioinformatics Division, TNLIST and Department of Automation, Tsinghua University, Beijing, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Dendritic Spines / metabolism*
Image Processing, Computer-Assisted / methods*
Microscopy, Confocal / methods*
Pattern Recognition, Automated / methods*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Electronic structure, chemical bonding, and geometry of pure and Sr-doped CaCO3.
Next Document:  A multi-model approach to simultaneous segmentation and classification of heterogeneous populations ...