Document Detail


Automatic classification of cells in cell cycle phases based on Ki-67 antigen quantification by fluorescence microscopy.
MedLine Citation:
PMID:  1932357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ki-67 antigen is thought to be a marker of cell proliferation, as it can be detected in cycling cells, i.e. cells in G1, S, G2 and M phases, but not in resting cells. The immunocytochemical staining pattern obtained by the Ki-67 monoclonal antibody varies, depending on the cell cycle phases. Analysis of double staining of Ki-67 antigen and DNA in the MCF-7 cell line by videomicrofluorometry allows the description of both the level and the pattern of Ki-67 staining in the form of a set of parameters defining each cell. These parameters were measured in MCF-7 cell populations characterized according to their position in the cell cycle. They were submitted to a statistical analysis (principal component and discriminant analysis) which allowed the determination of the optimal parameters to characterize a given cellular group and permitted the use of these parameters for an automatic classification of cells in the different cell cycle phases. In G1, S, G2, prophase + metaphase and anaphase + telophase cells, these parameters allowed a classification of cells with a good-classification rate of 94.37%. A comparison of this method with methods based on the DNA histogram and bromodeoxyuridine uptake was performed. The classification coefficients stemming from the discriminant analysis were introduced into a program to obtain, automatically, the Ki-67 labelling index and the percentages of cells in each phase. This method, which allows a quick evaluation of the proliferation and the phase indices, may be more widely applicable.
Authors:
P Guillaud; J Vermont; D Seigneurin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell proliferation     Volume:  24     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-11-26     Completed Date:  1991-11-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  481-91     Citation Subset:  IM    
Affiliation:
Equipe de Cytologie Quantitative, Faculté de Médecine de Grenoble, France.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms
Bromodeoxyuridine
Cell Cycle*
Data Interpretation, Statistical
Discriminant Analysis
Humans
Image Processing, Computer-Assisted
Ki-67 Antigen
Microscopy, Fluorescence
Nuclear Proteins / analysis*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Ki-67 Antigen; 0/Nuclear Proteins; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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