Document Detail


Automated solid-phase catalyzed pre-column derivatization of fatty acids for reversed-phase high-performance liquid chromatographic analysis with fluorescence detection.
MedLine Citation:
PMID:  3360885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described to automate the solid phase catalyzed reaction of a carbonyl group with labels containing a bromomethyl group. The acids to be determined must be soluble in an aprotic, non-aqueous reaction medium, such as acetonitrile. Special emphasis is placed on the solubility of the base, which serves as a catalyst. A suspension of potassium carbonate was prepared in acetonitrile. In this way, the effective surface area of the base is drastically enlarged resulting in faster reaction kinetics. The procedure is illustrated with the detection of fatty acids in serum and rat brain tissue with bromomethylmethoxycoumarin as label. The generality of our approach for HPLC determination of carbonyl compounds is discussed.
Authors:
J H Wolf; J Korf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  436     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-06-08     Completed Date:  1988-06-08     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  437-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoanalysis
Brain Chemistry
Chromatography, High Pressure Liquid
Fatty Acids / analysis*
Indicators and Reagents
Male
Rats
Rats, Inbred Strains
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Indicators and Reagents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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