| Automated solid-phase catalyzed pre-column derivatization of fatty acids for reversed-phase high-performance liquid chromatographic analysis with fluorescence detection. | |
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MedLine Citation:
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PMID: 3360885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method is described to automate the solid phase catalyzed reaction of a carbonyl group with labels containing a bromomethyl group. The acids to be determined must be soluble in an aprotic, non-aqueous reaction medium, such as acetonitrile. Special emphasis is placed on the solubility of the base, which serves as a catalyst. A suspension of potassium carbonate was prepared in acetonitrile. In this way, the effective surface area of the base is drastically enlarged resulting in faster reaction kinetics. The procedure is illustrated with the detection of fatty acids in serum and rat brain tissue with bromomethylmethoxycoumarin as label. The generality of our approach for HPLC determination of carbonyl compounds is discussed. |
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Authors:
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J H Wolf; J Korf |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 436 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1988 Feb |
Date Detail:
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Created Date: 1988-06-08 Completed Date: 1988-06-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: IM |
Affiliation:
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Department of Biological Psychiatry, University of Groningen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoanalysis Brain Chemistry Chromatography, High Pressure Liquid Fatty Acids / analysis* Indicators and Reagents Male Rats Rats, Inbred Strains Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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