Document Detail


Automated sample preparation and purification of homogenized brain tissues.
MedLine Citation:
PMID:  17451908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A robotic homogenized tissue sample transferring method has been developed by using a Packard MultiProbe II 8-tip system. It enables robotically transferring homogenized tissue samples from individual test tubes into a 96-well format plate for further sample purification and analysis. Extensive validation has been made to establish the accuracy and variability of this method. This automatic tissue sample transferring approach combined with automatic tissue homogenization, has significantly increased the throughput of tissue sample preparation in screening of drug candidates using liquid chromatography coupled with highly sensitive and selective tandem mass spectrometry (LC-MS/MS).
Authors:
S Xu; S Zheng; X Shen; Z Yao; J Pivnichny; X Tong
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Publication Detail:
Type:  Journal Article     Date:  2007-03-02
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  44     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-12     Completed Date:  2007-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  581-5     Citation Subset:  IM    
Affiliation:
Merck Research Laboratories, Merck & Co. Inc., Rahway, NJ 07065, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoanalysis
Brain Chemistry*
Chromatography, High Pressure Liquid
Mice
Rats
Reproducibility of Results
Robotics
Spectrophotometry, Ultraviolet
Tandem Mass Spectrometry

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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