Document Detail


Automated real-time measurement of chemotactic cell motility.
MedLine Citation:
PMID:  11730019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a novel method, (ECIS/taxis), for monitoring cell movement in response to chemotactic and chemokinetic factors. In this system, cells migrate in an under-agarose environment, and their positions are monitored using the electric cell-substrate impedance sensor technology to measure the impedance change at a target electrode, that is lithographed onto the substrate, as the cells arrive at the target. In the studies reported here, Dictyostelium discoideum was used as a prototypical, motile eukaryotic cell. Using the ECIS/taxis system, the arrival of cells at the target electrode was proportional to the dose offolate used to stimulate the cells and could be assessed by changes in resistance at the electrode. ECIS/taxis was readily able to distinguish between wild-type cells and a mutant that is deficient in its chemotactic response. Finally, we have shown that an agent that interferes with chemotactic motility leads to the delayed arrival of cells at the target electrode. The multi-well assay configuration allows for simultaneous automated screening of many samples for chemotactic or anti-chemotactic activity. This assay system is compatible with measurements of mammalian cell movement and should be valuable in the assessment of both agonists and antagonists of cell movement.
Authors:
N Hadjout; G Laevsky; D A Knecht; M A Lynes
Related Documents :
25412289 - The combination of arsenic and cryptotanshinone induces apoptosis through induction of ...
3779849 - Multiple forms of acid phosphatase and their differential secretion during stalk format...
22438279 - Characterization of plasmodium liver stage inhibition by halofuginone.
22753749 - In vitro cytotoxic activity of novel protoflavone analogs - selectivity towards a multi...
11959449 - Active and energy-dependent rapid formation of cell aggregates in the thermophilic phot...
11572999 - Fruiting body formation by bacillus subtilis.
16186709 - Cryopreservation of mature human adipocytes: in vitro measurement of viability.
8856739 - Characterization of the mdck cell line for screening neurotoxicants.
9242269 - Anticipatory time intervals of head-direction cells in the anterior thalamus of the rat...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  BioTechniques     Volume:  31     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-03     Completed Date:  2002-04-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1130-8     Citation Subset:  IM    
Affiliation:
University of Connecticut, Storrs 06269, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Movement / drug effects
Chemotaxis*
Cisplatin / pharmacology
Dictyostelium / physiology
Dose-Response Relationship, Drug
Electric Impedance
Folic Acid / pharmacology
Grant Support
ID/Acronym/Agency:
AI46790/AI/NIAID NIH HHS; GM40599/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
15663-27-1/Cisplatin; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Interrogation of multimeric DNA amplification products by competitive primer extension using bst DNA...
Next Document:  Under-agarose folate chemotaxis of Dictyostelium discoideum amoebae in permissive and mechanically i...