Document Detail

Automated rapid screening of foods for the presence of salmonellae.
MedLine Citation:
PMID:  10571327     Owner:  NLM     Status:  MEDLINE    
We previously reported an accelerated method to detect salmonellae in foods. It consisted of a preenrichment step (6 h) followed by immunomagnetic separation (IMS) and automated detection, based on positive biochemical characteristics, during overnight incubation. We describe here a simplified procedure with a detection limit of 2 CFU/25 g of food, a procedure that does not require the IMS step. Liquid eggs, milk, ice cream, or poultry were inoculated with Salmonella or non-Salmonella Enterobacteriaceae organisms (2 to 100 CFU/25 g). Following preenrichment for 6 h at 35 degrees C, samples (3 ml) were added to selective liquid media (6 ml). Automated light transmittance was recorded during incubation at 42 degrees C in the BioSys instrument. Salmonella-positive samples were identified by a sharp drop in transmittance (caused by black discoloration of the media) within 18 h of incubation following the formation of hydrogen sulfide and its reaction with iron ions. Detection of 2 to 10 CFU/25 g of the tested foods was completed in 24 h. A total of 24 Salmonella spp., including heat-injured cells, and 37 non-Salmonella Enterobacteriaceae were tested using the procedure.
H Peng; L A Shelef
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  62     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-21     Completed Date:  1999-12-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1341-5     Citation Subset:  IM    
Department of Nutrition and Food Science, Wayne State University, Detroit, Michigan 48202, USA.
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MeSH Terms
Enterobacteriaceae / isolation & purification
Food Microbiology*
Hot Temperature
Salmonella / isolation & purification*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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